FEATURES OF GLUCOSE METABOLISM IN HORMON-SENSITIVE AND RESISTANT HUMAN PROSTATE CANCER CELLS IN VITRO
DOI:
https://doi.org/10.32471/oncology.2663-7928.t-23-4-2021-g.9925Keywords:
glucose, hormone- resistant, hormone-sensitive, prostate cancer, інсулінAbstract
Prostate cancer (PC) is less common in patients with diabetes. However, in combination with diabetes, PC is more aggressive and accompanied by metastasis to regional lymph nodes. Aim: to study the effect of insulin on the proliferation and glucose metabolism in PC cells. Object and Methods: the study was performed in vitro on human PC cell lines LNCaP (hormone-sensitive), Du-145, and PC-3 (resistant). The methods of cell culture, biochemical, molecular biological, immunological, and statistical methods were used in the study. Results: insulin increased glucose metabolism in LNCaP cells by 20% in all studied concentrations. However, in Du-145 cells, only high concentrations of insulin significantly increased the amount of metabolized glucose. Insulin increased the proliferation of LNCaP cells by 20–30% in all studied concentrations, but in Du-145 and PC-3 cells, high concentrations of the hormone led to inhibition of cell proliferation. Treatment of PC cells with insulin resulted in dose-dependent inhibition of CD44 expression in all three cell types. Conclusions: comparative analysis of the obtained results shows that the increase in the degree of PC cells malignancy is associated with a decrease in their sensitivity to the metabolic and mitogenic effects of insulin. CD44 can be an important and selective molecular target for the treatment of PC.
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