STUDY OF INFECTION BY HERPES VIRUSES (CMV, EBV) OF VARIOUS NATURE BRAIN TUMORS
Keywords:
brain tumors, cytomegalovirus, Epstein — Barr virus, tumor tissue, blood, polymerase chain reaction.Abstract
Summary. Objective: to study the frequency of persistence of cytomegalovirus (CMV) and Epstein — Barr
virus (EBV) in tissue of different histogenesis and degree of malignancy of brain tumors, as well as in the
blood of neuro-oncological patients. Object and methods: we investigated 231 sample biopsy/surgical material of various brain tumors and 60 samples of whole peripheral blood of patients (127 adults, 14 children aged
1–15 years) who underwent surgery in 2014–2015 at
the SI «A.P. Romodanov Institute of Neurosurgery of
NAMS of Ukraine». Histological structure of the tumors was studied according to the standard technique,
using the modern classification. The detection in tumor
tissue and blood for CMV and EBV was performed by
a conventional method, the polymerase chain reaction
(PCR) real-time. DNA extraction from samples was
performed using sets of «DNA Sorb-B» («AmpliSens»,
Russia) according to the instructions of the manufacturer. PCR was carried out using the device Bio Rаd, with
diagnostic kits to detect CMV and EBV (DNA-technology, Russia) according to the instructions. Statistical processing of results was performed using the program «Statistica 9» with the definition of Pearson’s χ2
and index p. Results: the most frequently detected DNA
of CMV and EBV in tissue glial intracerebral tumors at
33.3 and 41.3%, respectively. Among connective tissue
tumors of the cerebral meninges (meningiomas) of positive samples was significantly lower (p < 0.05); medulloblastoma and other tumors took an intermediate place.
In differentiated glial tumor DNA of both viruses was
identified in the 2.2–2.5 times more often than in glioblastomas IV degree of malignancy. In whole peripheral
blood DNA CMV was detected in 6.2%, DNA EBV —
in 30.8% of cases. Conclusion: the frequency of the presence of CMV DNA and EBV in brain tumors depend on
their histogenesis and degree of malignancy, significantly higher than that in whole peripheral blood of patients.
References
Васильева ИГ. Биомолекулярные механизмы развития глиом. В: Глиомы головного мозга. Под ред.: Ю.А. Зозули. Киев, 2007: 35–91.
Лисяный НИ, Орлов ЮА, Кулик АВ и др. Дагностика вирус-ассоциированных опухолей головного мозга. Укр нейрохирург журн 2008; (1): 27–31.
Cobbs С, Harkins S, Santana V. Нuman cytomegalovirus infection and expression in human malignant gliomas. Cancer 2002; 62: 3347–50.
Scheurer ME, Bondy ML, Aldape KD, et al. Detection of human cytomegalovirus in different histological types of gliomas. Acta Neuropathol 2008; 116: 79–86.
Moore PS, Chang Y. Why do viruses cause cancer? Highlights of the first century of human tumours virology. Nat Rev Cancer 2010; 10: 878–89.
Soroceanu L, Cobbs CS. Is HCMV a tumor promoter? Virus Res 2011; 157: 193–203.
Cinatl J Jr, Vogel JU, Kotchetkov R, еt al. Oncomodulatory signals by regulatory proteins encoded by human cytomegalovirus: a novel role for viral infection in tumour progression. FEMS Microbiol Rev 2004; 28 (1): 59–77.
Poltermann S, Schlehofer B, Steindorf K, et al. Lack of association of herpesviruses with brain tumor. J Neurovirol 2006; 12 (2): 90–9.
Lau SK, Chen YY, Chen WG, et al. Lack of association of cytomegalovirus with human brain tumors. Mod Pathol 2005; 18 (6): 838–43.
Лисяный АН. Выявление антител к цитомегаловирусу в сыворотке крови и рр65 этого вируса в непластических клетках больных с опухолями головного мозга. Онкология 2013; 15 (2): 108–11.
Baryawno N, Rahbar A, Wolmer-Solberg N. Detection of human cytomegalovirus in medulloblastomas reveals a potential therapeutic target. J Clin Invest 2011; 21 (10): 4043–55.
Johnsen JI, Baryawno N, Soderberg-Naucler C, et al. Is human cytomegalovirus a target in cancer therapy? Oncotarget 2011; 2 (12): 1329–38.
Лисяный НИ, Ключникова АИ, Лисяный АН и др. Частота выявления ДНК герпеса 4 типа в опухолях головного
мозга. Укр нейрохирург журн 2015; (3): 35–7.
Louis DN, Ohgaki H, Wiestler OO, et al. The 2007 WHO classification of tumours of the central nervous system. Acta Neuropatol 2007; 114 (2): 97–109.
Shen Y, Zhu H, Shenk T. Human cytomegalovirus IE1 and IE2 proteins are mutagenic and mediate «hit-and-run» oncogenic transformation in cooperation with the adenovirus E1A proteins.Proc Natl Acad Sci U S A 1997; 94 (7): 3341–5.
Лисяный НИ, Гнедкова ИА, Станецкая ДН и др. Связь цитомегаловируса со злокачественными глиомами мозга. Укр нейрохирург журн 2016; (1): 44–9.
Лисяный НИ, Станецкая ДН, Гнедкова ИА и др. Особенности иммунных нарушений при глиобластомах, инфицированных цитомегаловирусом. Імунологія та алергологія: наука та практика 2015; (2): 5–11.
