PARTIAL PRESSURE OF GASES AND LACTATE LEVEL IN RATS BLOOD WITH SENSITIVE AND DRUG-RESISTANT MALIGNANT TUMORS
DOI:
https://doi.org/10.32471/oncology.2663-7928.t-23-3-2021-g.9710Keywords:
blood gases, drug resistance, lactate level, partial pressure, tumorAbstract
Aim: determination of partial pressure of gases and lactate level in the blood of the rats with sensitive and cytostatic-resistant malignant tumors. Objects and methods: the study was carried out in Wistar female rats weighed 230–250 g. The rats were inoculated subcutaneously with sensitive and cisplatin-resistant Guerin carcinoma and sensitive and doxorubicin-resistant carcinosarcoma Walker-256. pH, pO2, pCO2 and lactate level were determined in the whole blood at exponential growth (the 9th day after carcinosarcoma Walker-256 and the 13th day after Guerin carcinoma inoculation). The determination of these indicators was carried out using blood gas system ABL800 FLEX («Radiometer», Denmark). For the data statistical analysis Student’s coefficient was used. Results: the pH and pCO2 in the blood of the animals with resistant tumors doesn’t differ from the one of the rats either with a sensitive tumor or of the intact rats. Nonetheless, pO2 in the blood of the animals with the resistant tumors was statistically lower comparing to the rats with the sensitive tumor. The lactate level in the blood of the animals with the tumor (either sensitive or resistant) was higher comparing to the intact rats. The identified patterns were the same for both experimental models of tumor growth. Conclusion: drug-resistant tumors cause more negative influence on the organism oxygenation.
References
Lundholm K, Sylund A, Holm G. Skeletal muscle metabolism in patients with malignant tumor. Eur J Cancer 1976; 12 (6): 465–73.
Waterhouse C. Lactate metabolism in patients with cancer. Cancer 1974; 33 (1): 66–71.
Weber G, Kizaki H, Shiotani T. Biochemical strategy of hepatomas. J Toxicol Environ Health 1979; 5 (2–3): 371–86.
Rundqvist H, Johnson RS. Tumour oxygenation: implications for breast cancer prognosis. J Intern Med 2013; 274: 105–11.
Muz B, de la Puente P, Azab F. The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia 2015; 3: 83–92.
Bertout JA, Patel SA, Simon MS. The impact of O2 availability on human cancer. Nat Rev Cancer 2008; 8 (12): 467–75.
Sorensen BS, Horsman MR. Tumor hypoxia: impact on radiation therapy and molecular pathways. Front Oncol 2020; 10: 562. doi:10.3389/2020.00562.
Cao X, Allu RS, Jiang S, et al. Tissue pO2 distributions in xenograft tumors dynamically imaged by Cherenkov-excited phosphorescence during fractionated radiation therapy. Nature Com 2020; 11: 573. doi.org/10.1038/s41467–020–14415–9.
Barcellos-Hoff MH, Nguyen DH. Radiation canrcinogenesis in context: how do irradiated tissues become tumors? Health Phys 2009; 97 (5): 446–57. doi:10.1097/HP.0b.013e3181b08a10.
