THE INFLUENCE OF SPERMINE ON THE SURVIVAL AND ELECTROKINETIC CHARACTERISTICS OF HUMAN PROSTATE CANCER LNCaP CELL LINE
DOI:
https://doi.org/10.32471/oncology.2663-7928.t-24-3-2022-g.10728Keywords:
aminoguanidine, LNCaP cell line, spermine, total surface charge, ζ-potentialAbstract
Aim: study the relationship between the survival of human prostate cancer LNCaP cells, their total surface charge and ζ-potential in the presence of an excess of spermine or putrescine in the nutrient medium and to determine the half-inhibitory concentration IC50 of spermine. Object and methods: the study was conducted using human prostate cancer cells of the LNCaP cell line. Vitality was determined in cell samples, with total number 0.1–1.5•106. Cells were treated with spermine, putrescine, and aminoguanidine at concentration range 0.01–10 mM. The IC50 inhibitory dose was determined using the Quest Graph™ IC50 program (AAT Bioquest, USA). The electrokinetic, or ζ-potential, was calculated according to the equation of M.R. Smolukhovskyi. The density of the total surface charge of the cells was calculated according to the Quincke-Helmholtz equation. Results: the protective properties of aminoguanidine against the inhibitory effect of spermine were demonstrated, if the concentration of the spermine equals or exceeds the IC50. It has been shown that spermine in concentrations of 1.5 mM and higher causes the death of prostate cancer cells in vitro and can be considered as a potential antitumor agent. Conclusion: the main result of this study is the establishment of a clear inhibitory and antiproliferative effect of spermine in certain concentrations in relation to the number of cells. It was shown that alongside with decreasing of cell vitality under the influence of spermine, there was observed a change in the sign of their surface charge from negative to positive.
References
Yanish YuV, Zaletok SP, Klenov OO, et al. Electrokinetic characteristics of leukemic cells. Oncologia 2020; 22 (1–2): 60–5. doi: 10.32471/oncology.2663-7928.t-22-1-2020-g.8728 (in Ukrainian).
Yanish YuV, Zaletok SP, Bentrad VV, Sklyarenco LM. Electrokinetic characteristics of lymphoid cells of cancer patients and healthy donors. Clin Oncol 2018; 8 (3): 216–9 (in Ukrainian).
Akagi T, Kato K, Hanamura N, et al. Evaluation of desialylation effect on zeta potential of extracellular vesicles secreted from human prostate cancer cells by on-chip microcapillary electrophoresis. Jap J of Appl Phys 2014; 53: 06JL01.
Zaletok SP, Klenov OO, Bentrad VV, et al. Polyamines as new potential biomarkers for differential diagnosis of prostate cancer and estimation of its aggressiveness. Exp Oncol 2022; 44 (2): 148–54. doi: 10.32471/exp-oncology.2312-8852.vol-44-no-2.17758.
Yanish YuV, Zaletok SP, Vityuk NV, Mukha YuP. Application of gold and silver nanoparticles for selective assay of spermine in mixture with spermidine. Exp Oncol 2021; 43 (1): 77–81. doi:10.32471/exp-oncology.2312-8852.vol-43-no-1.15609.
Harms M, von Maltitz P, Grob R, et al. Utilization of aminoguanidine prevents cytotoxic of semen. Int J Mol Sci 2022; 23: 8563. doi: 10.3390/ijms23158563.
Seiler N, Knodgen В, Bink G, et al. Diamine oxidase and catabolism polyamines. Advances in polyamine research 1999. N.Y.: Raven press: 412−30.
Buckingham L, Hao T, O’Donnell J, et al. Ipatasertib, an oral AKT inhibitor, inhibits cell proliferation and migration, and induces apoptosis in serous endometrial cancer. Am J Cancer Res 2022; 12 (6): 2850–62. PMID: 35812065.
Ghasemi A, Salari A, Kalantarmahdavi M, Amiryousefi M. Sodium metabisulfite in dried plum and its cytotoxic effects on K-562 and L-929 normal cell lines. J Food Sci 2022; 87: 856–66. doi: 10.1111/1750-3841.16034.
Smoluchowski MR. About Brownian molecular motion under action of external forces and their connection with the generalized diffusion equation. Ann Phys 1915; 48: 1103 (in German).
Jensen G-L. Electrophoresis of cells. Immunological methods (ed. H. Frimel). M: Mir, 1979. 296 p (in Russian).
Lakin GF. Biometrics. M: Higher School, 1990. 352 p (in Russian).
Del Rio B, Redruello B, Linares DM, et al. The biogenic amines putrescine and cadaverine show in vitro cytotoxicity at concentrations that can be found in foods. Sci Rep 2019; 9 (1): 1–7. doi: 10.1038/s41598-018-36239-w.
