EXPRESSION PROFILE OF TRANSCRIPTION FACTORS IN THE BLOOD SAMPLES OF PATIENTS WITH THE CHRONIC LYMPHOCYTIC LEUKEMIA
Keywords:
chronic lymphocytic leukemia, transcription factors, gene expression profile.Abstract
Summary. Aim: to investigate the expression profile
of transcription factors in chronic lymphocytic leukemia
(CLL) cells compared with B cells to identify mechanisms
of CLL appearance. Objects and methods: Samples of peripheral blood of patients with CLL, RNA isolation, analysis of expression of transcription factors, using RT2
profiler Array and quantitative polymerase chain reaction. Results: using the PARN-075Z platform, expression of the 84
transcription factors was studied in a mixture of RNA isolated from CLL cells of patients, in comparison B cells and
a mixture of RNA isolated from B- and T-cells of healthy
donors. We have found that genes POU2ATF1, NFAT5,
NFATC1, JUNB, JUN, and RELB were expressed at higher levels in a mixture of RNA from CLL cells when compared with B-cells. Using quantitative polymerase chain
reaction, we have observed that genes HAND1, HOXA5,
and SMAD9 were similarly expressed in CLL cells of individual patients while the ID1 gene, in contrast, showed
a higher expression compared with normal B-cells. Conclusions: the expression of the 84 transcription factors was
studied in a mixture of RNA isolated from cells of CLL
patients, compared with B cells from healthy donors. Given the heterogeneity of patients, gene expression was analyzed in samples of individual patients. It was confirmed
that genes HAND1, HOXA5, and SMAD9 expressed at low
levels in CLL cells of individual patients, unlike genes ID1
and JUNB that showed a heterogeneous pattern of expression. A clear difference in expression profiles allows us to
extend our research to identify cellular pathways blocked
in CLL cells, using bioinformatics analysis.
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