THE INFLUENCE OF ROS-GENERATING SYSTEMS ON OXIDATIVE METABOLISM OF INTACT AND TUMOR BEARING MICE
Abstract
Summary. An imbalance of reactive oxygen species
(ROS) generation in cells and deregulation of antioxidant systems is the trigger of oxidative metabolism disorders in organism. Objective: to investigate the effect
ofROS-generating systems on oxidative metabolism and
antitumor activity of macrophages. Object and methods:
the study was conducted on the C57Bl/6 mice with Lewis
lung carcinoma. The animals were treated with single or
fractionated ROS-generating agents (ascorbic acid, vitamin K, ornithine copper complex, ferrocene, hydrogen
peroxide). We used biochemical, biophysical, spectrometric and statistical methods. Results: studying factors
activate the generation of superoxide anion radical by
macrophages and inhibit its production by hepatocytes.
The reduction of catalase activity in the liver and tumor
cells was observed. However, in the blood we detected
a tendency to it activation after treatment with pairs of
investigated factors. Conclusions: the maximum effect
of a course use of ROS-generating systems reported after combined administration of ascorbic acid and copper that can be used for the development of anticancer
drugs. Course use of ROS-generating systems (in particular ferrocene, and hydrogen peroxide) violated oxidative metabolism in the Lewis lung carcinoma cells,
which is accompanied by a decrease in catalase activity.
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