CHANGES IN THE RANGE OF METALLOPROTEINS AND THEIR REGULATORY FACTORS IN GROWTH DYNAMIC OF SENSITIVE AND RESISTANT GUERIN CARCINOMA
Keywords:
iron, microRNA, methylation, Guerin carcinoma, resistance, growth dynamics.Abstract
Summary. Aim: to analyze changes in the range of
metal-containing proteins and certain factors of their
epigenetic regulation in serum and tumor tissue of animals in dynamics of growth of sensitive and resistant
Guerin carcinoma (GC). Subject and methods: the
study was conducted on rats with sensitive and resistant to doxorubicin and cisplatin GC. Levels of ferritin, transferrin, ceruloplasmin and free iron complexes were measured in tumor tissue and blood serum using ELISA and low-temperature EPR. fth1 and
tfr1 promoters methylation and expression levels of
microRNA-122, -133a, -200b and -320a were evaluated using RT-PCR. Results: we showed that the dynamics of growth of GC is accompanied by significant changes in expression and epigenetic regulation
of metal-containing proteins in tumor tissue and serum. Also we determined differences which allowed
to differentiate sensitive and resistant to doxorubicin and cisplatin tumors, as well as stages of their
growth. Conclusions: obtained data showed the important role of metal-containing proteins and their regulation in the process of tumor growth and development of resistance to anticancer drugs. These results
can be used to improve differential diagnosis of tumors and to assess their sensitivity to cytotoxic drugs.
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