CONTENT OF NEOPLASTIC STEM CD133+ AND CD15+ CELLS IN TUMORS AND LYMPHOCYTES IN PERIPHERAL BLOOD OF PATIENTS WITH GLIOMAS OF DIFFERENT LEVEL ANAPLASIA
Keywords:
stem cells glioma, CD133, CD15, lymphocytes, immunodepression.Abstract
Summary. Aim of this study was to investigate the content
of stem tumor cells CD133+ and CD15+ in the gliomas depending on the degree of anaplasia tumor and to compare
in percentage and absolute content lymphocytes in peripheral blood of these patients. Objects and methods: bioptic material of 79 gliomas: low level astrocytomas II grade — 17;
anaplastic astrocytomas III grade — 28 and glioblastomas
IV grade — 34 was studied. Phenotype of tumor cells was
estimated by means of immunofruorescent method on cytofluorometr («Beckman Coulter», USA) according to protocol. Phenotype of tumor cells was determined by means of
monoclonal antibody (mAb) to molecular CD133 («Millipore») and CD15FITC («Becton Dickinson», USA). Ordinary methods were used for examine absolute and in percentage content of lymphocytes in peripheral blood of these
patients. Results: the results showed two types cells CD133
and CD15 in gliomas. Their content increases with growth
of level of anaplasia gliomas. The content of CD15 cells in
every type of glioma was twice more than it was for CD133
cells. It was established a significant degree in percentage
and absolute content of lymphocytes in peripheral blood with
growth ofthe level anaplasia. The most of content ofCD133
and CD15 cells was noted upon prolong growth of malignant
gliomas (III–IV) which distinguished a significant level of
all content of lymphocytes in peripheral blood. It was supposed the similarity ofCD15 receptors tumor and immunocompetent cells causes the suppression of immune answer on
tumor cells and the development of immunosupression. Conclusion: one of the mechanism development of immunosupression upon gliomas is the increase of content and activation of stem gliomas cells. On another side, the development
of immunodepression may causes the activity of proliferation of stem cells too. The study of the interaction stem cells
glioma and immunocompetent cells will be useful for working out the new approaches in immunotherapy.
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