THE REDOX-STATE INDICES OF BREAST ADIPOSE TISSUE IS IMPORTANT FACTOR OF TUMOR MICROENVIRONMENT IN PATIENTS WITH BREAST CANCER
DOI:
https://doi.org/10.32471/oncology.2663-7928.t-21-2-2019-g.7181Abstract
Summary. Breast adipose tissue (BAT) is a heterogeneous population of cells, which is represented by adpocytes and their precursors, endothelial cells, macrophages, pericytes. Due to the diversity of functions of
superoxide radicals (SR), nitric oxide (NO), steroid hormones produced by its cells, BAT can affect the development and progression of breast cancer(BC). Objectives: the study of the levels of redox state indices of BAT in patients with triple negative (TN) BC with type II diabetes mellitus. Materials and methods: the samples of adjacent to the BAT (1 cm) were studied in 39 patients with TN BC II–III stage with normoglycaemia (n = 23) and type 2 diabetes mellitus (n = 16). According to the indicator of the ratio of the BAT volume to the total breast (7 ÷ 56%, median 21%) the patients were divided into 2 groups: the BAT volume < 21% and > 21%. Determination of the activity of cytochrome P450, the content of NO and the rate of generation of SR in BAT was carried out by the method of electron paramagnetic resonance. The level of oxidative damages of DNA (8-охоdGu) in BAT was determined spectrophotometrically. The level of adiponectin expression was detected by immunohistochemistry. Results: in patients with TN BC (normoglycaemia) with BAT volume > 21%, the increase in the rate of SR generation, the level of oxidative DNA damage and the total activity of cytochrome P450 were determined, with a decrease in the level of expression of adiponectin (1.7 times) and NO compared to patients with BAT < 21%. In patients with BC with a violation of carbohydrate metabolism (type 2 diabetes mellitus)
with BAT > 21%, an increase in the rate of SR generation, level of oxidative DNA damage, activation of the
detoxification system (cytochrome P450), reduction of adiponectin and NO levels, compared to patients with BAT < 21% were observed. Conclusions: violations of the ratio of hormonal and genotoxic parameters in the
BAT, which are defined as local (placement of adipocyte tissue in relation to the tumor) and systemic factors
(carbohydrate metabolism disturbance), were revealed.
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