DETERMINATION OF EFFECTS AND OPTIMIZATION OF PHOTOBIOMODULATION AND PHOTODYNAMIC THERAPY PARAMETERS FOR PROSTATE AND BREAST CANCER CELL LINES OF DIFFERENT MALIGNANCY GRADE
DOI:
https://doi.org/10.32471/oncology.2663-7928.t-23-4-2021-g.9946Keywords:
prostate cancer, breast cancer, cell lines, laser irradiation, photodynamic therapy, photobiomodulation, doxorubicin.Abstract
Summary. Aim: to investigate the effects of laser radiation of different intensities on prostate cancer (PC)and breast cancer (BC) cell lines; to evaluate the prospects of combining light stimulation (photobiomodulation) and chemotherapy for synergistic effects on tumor cells. Object and methods: PC cell lines of different malignancy grade (LNCaP and DU-145) and BC
cell line (MCF-7) were used in the study. The radiation of the semiconductor laser device (Photonics Plus,
Ukraine) with wavelengths of 405, 445, 660, 635 and
810 nm was used to irradiate cells. The power of light
radiation was measured using an optical radiation
meter Ophir Optronics (USA). As the photosensitizer (PHS), 5-aminolevulinic acid (5-ALA) (1 mmol)
was applied to LNCaP and DU-145 cells; doxorubicin (DOX) at a concentration of 1 μg/ml was added to
the MCF-7 cells. The statistical analysis of the results
was performed with the software package Statistics 6.0.
Results: It has been found out on PC cells lines (LNCaP and DU-145) that the most optimal parameters of
laser radiation for obtaining the highest photodynamic
activity of 5-ALA are as follows: wavelength 405 nm,
irradiation time 10 min, power density 20 mV/cm2
. It
has been shown that the cytotoxic effect of DOX towards BC cells (MCF-7) is enhanced by laser radiation with the wavelength of 445 nm, which is equal to
the peak of DOX absorption. Additionally, used as photobiomodulation, laser radiation with a wavelength of
810 nm before the administration of chemotherapeutical drug can increase its overall toxic effect on MCF7 cells. Conclusions: the use of laser radiation with the
wavelength which is equivalent to the chemotherapeutical drug absorption peak can increase its toxic effects
without increasing the dose and irradiation time. The
additional application of laser radiation for photobiomodulation increases the overall toxic effect of a chemotherapeutic agent towards tumor cells.
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