REDOX-DEPENDENT FEATURES OF TUMORS, ADIPOSE TISSUE, NEUTROPHILES AND PLATELETS IN PATIENTS WITH COLORECTAL CANCER
DOI:
https://doi.org/10.32471/oncology.2663-7928.t-24-1-2022-g.10237Keywords:
adipose tissue, colorectal cancer, matrix metalloproteinases, neutrophils, platelets, superoxide radicalsAbstract
Aim: to study the features of superoxide radicals (SOR) generation, the content of reactive nitric oxide (NO) and free fatty acids, the activity of matrix metalloproteinases-2 and -9 (or gelatinases) in tumor and adipose tissue cells, as well as in blood neutrophils and platelets in patients with colorectal cancer in the overweight. Objects and methods: Electron paramagnetic resonance spectrometry, zymography in polyacrylamide gel, biochemical, spectrophotometric and statistical methods were used. Results: it was revealed that the accumulation of dysfunctional adipose tissue in a patient’s body promotes an increase in the rate of generation of superoxide radicals in the tumor, superoxide-generating activity of neutrophils and platelets, and the activity of matrix metalloproteinases. Conclusions: imbalance in the redox state of neutrophils and platelets elicited by dysregulated SOR formation as well as hyperlipidemia and activation of metalloproteinases in the overweight can modify tumor microenvironment that in turn promotes the formation of a highly invasive and aggressive cancer cells phenotype as well as facilitates metastasizing due to increased efficacy of tumor cells dissemination.
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