Study of products of the interaction of RNA with mannitol, by using the pyrolytic mass spectrometry method

Authors

  • V.B. Shchodryi Institute of Molecular Biology and Genetics of the NAS of Ukraine, Kiev
  • O.V. Kozlov Institute of Molecular Biology and Genetics of the NAS of Ukraine, Kiev
  • A.O. Rybenchuk Institute of Molecular Biology and Genetics of the NAS of Ukraine, Kiev
  • V.V. Boyko Institute of Macromolecular Chemistry of the NAS of Ukraine, Kiev
  • V.I. Bortnitskiy Institute of Macromolecular Chemistry of the NAS of Ukraine, Kiev
  • S.V. Rybov Institute of Macromolecular Chemistry of the NAS of Ukraine, Kiev
  • Z.Yu. Tkachuk Institute of Molecular Biology and Genetics of the NAS of Ukraine, Kiev

DOI:

https://doi.org/10.15407/dopovidi2017.02.079

Keywords:

mannitol, mass-spectrometry, Nuclex, RNA

Abstract

We studied the structural features of the molecular structure of a complex of RNA and mannitol by means of thermal mass spectrometry. It is shown that the mass spectra of RNA and the complex, which is the basis of medicine Nuclex, are similar and characterized by two peaks. They consist of the same ion fragments, but their relative intensity in the case of Nuclex is higher than in RNA. However, Nuclex has the fragment third in ion intensity with m/z = 86 (C3H6N2O), which is absent in the mass spectra of the original substances. There is a reason to believe that it is a product of the interaction of mannitol and nitrogenous bases of RNA. Increasing the weight ratio of RNA in relation to mannitol leads to a significant reduction in the number of volatile products specific to the first thermal peak and to a drastic increase in volatile products of the second peak.

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References

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Vivcharyk, M. M., Ilchenko, A. A., Levchenko, S. M., Tkachuk, S. Yu. (2016). Reports of the National Academy of Sciences of Ukraine, No 10, pp. 78-83 (in Ukrainian). https://doi.org/10.15407/dopovidi2016.10.078

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Published

22.05.2024

How to Cite

Shchodryi, V., Kozlov, O., Rybenchuk, A., Boyko, V., Bortnitskiy, V., Rybov, S., & Tkachuk, Z. (2024). Study of products of the interaction of RNA with mannitol, by using the pyrolytic mass spectrometry method . Reports of the National Academy of Sciences of Ukraine, (2), 79–87. https://doi.org/10.15407/dopovidi2017.02.079

Issue

No. 2 (2017): Reports of the National Academy of Sciences of Ukraine

Section

Biology

License

Copyright (c) 2024 Reports of the National Academy of Sciences of Ukraine

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