Mass spectrometric analysis of oligoribonucleotides of total yeast RNA
DOI:
https://doi.org/10.15407/dopovidi2021.02.115Keywords:
yeast RNA, ribonucleotides, liquid chromatography, MALDI-Tof-Tof mass spectrometryAbstract
To find out the composition of oligoribonucleotides of total yeast RNA ingredient that is present in antiviral drug Nuclex with multiple actions and immunomodulator Nucleinat, are liquid chromatography and mass spectrometry MALDI-Tof-Tof methods were used for ribonucleotides analysis. It was shown that the total yeast RNA contains three main fractions: the first one — from 2 to 4 nt, the second one — from 4 to7 nt and the third one — from 10 to 15 nt. It is shown that the total yeast RNA contains different oligoribonucleotides with lengths from 4 to 15 nt.
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Sullenger, B.A. & Gilboa, E. (2002). Emerging clinical applications of RNA. Nature, 418, pp. 252-258. https://doi.org/10.1038/418252a
Biesecker, G., Dihel, L., Enney, K. & Bendele, R.A. (1999). Derivation of RNA aptamer inhibitors of human complement C5. Immunopharmacology, 42, № 1-3, pp. 219-230. https://doi.org/10.1016/s0162-3109(99)00020-x
Sundaram, P., Kurinawan, H., Byrne, M.E. & Wower, J. (2013). Therapeutic RNA aptamers in clinical trials. Eur. J. Pharm. Sci., 48, pp. 259-271. https://doi.org/10.1016/j.ejps.2012.10.014
Burnett, J.C. & Rossi, J.J. (2012). RNA-based therapeutics: current progress and future prospects. Chem. Biol., 19, pp. 60-71. https://doi.org/10.1016/j.chembiol.2011.12.008
Pat. 8722642 B2 US, Multiantivirus compound, composition and method for treatment of virus diseases, Tkachuk, Z. Publ. 13.05.2014.
Keefe, A.D., Pai, S. & Ellington, A. (2010). Aptamers as therapeutics. Nat. Rev. Drug Discov., 9, pp. 537-550. https://doi.org/10.1038/nrd3141
Thomas, B. & Akoulitchev, A.V. (2006). Mass spectrometry of RNA. Trends Biochem. Sci., 31, № 3, pp. 173-181. https://doi.org/10.1016/j.tibs.2006.01.004
Banoub, J.H., Newton, R.P., Esmans, E., Ewing, D.F. & Mackenzie, G. (2005). Recent developments in mass spectrometry for the characterization of nucleosides, nucleotides, oligonucleotides, and nucleic acids. Chem. Rev., 105, № 5, pp. 1869-1916. https://doi.org/10.1021/cr030040w
Chiang, C.-K., Yang, Z., Lin, Y.-W., Chen, W.-T., Lin, H.-J. & Chang H.-T. (2010). Detection of proteins and protein−ligand complexes using HgTe nanostructure matrixes in surface-assisted laser desorption/ionization mass spectrometry. Anal. Chem., 82, № 11, pp. 4543-4550. https://doi.org/10.1021/ac100550c
Jiménez, C.R., Huang, L., Qiu, Y. & Burlingame A.L. (2001). Sample preparation for MALDI mass analysis of peptides and proteins. Curr. Protoc. Protein Sci., Chapter 16, Unit 16.3. https://doi.org/10.1002/0471140864.ps1603s14
Apffel, A., Chakel, J.A., Fischer, S., Lichtenwalter, K. & Hancock, W.S. (1997). Analysis of oligonucleotides by HPLC-electrospray ionization mass spectrometry. Anal. Chem., 69, № 7, pp. 1320-1325. https://doi.org/10.1021/ac960916h
Huth-Fehre, T., Gosine, J.N., Wu, K.J. & Becker, C.H. (1992). Matrix-assisted laser desorption mass spectrometry of oligodeoxythmidylic acids. Rapid Commun. Mass Spectrom., 6, № 3, pp. 209-213. https://doi.org/10.1002/rcm.1290060312
Nordhoff, E., Ingendoh, A., Cramer, R., Overberg, A., Stahl, B., Karas, M., Hillenkamp, F., Crain, P.F. & Chait, B. (1992). Matrix-assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared. Rapid Commun. Mass Spectrom., 6, № 12, pp. 771-776. https://doi.org/10.1002/rcm.1290061212
Pieles, U., Zürcher, W., Schär, M. & Moser, H.E. (1993). Matrix-assisted laser desorption ionization time-offlight mass spectrometry: a powerful tool for the mass and sequence analysis of natural and modified oligonucleotides. Nucleic Acids Res., 21, № 14, pp. 3191-3196. https://doi.org/10.1093/nar/21.14.3191
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