Complexation of RNA with mannitol, its spectral characteristics and biological activity

Authors

  • M.M. Vivcharyk Institute of Molecular Biology and Genetics of the NAS of Ukraine, Kyiv
  • O.O. Ilchenko Taras Shevchenko National University of Kyiv
  • S.M. Levchenko Institute of Molecular Biology and Genetics of the NAS of Ukraine, Kyiv
  • Z.Yu. Tkachuk Institute of Molecular Biology and Genetics of the NAS of Ukraine, Kyiv

DOI:

https://doi.org/10.15407/dopovidi2016.10.078

Keywords:

T-Raman spectroscopy, FTIR spectroscopy, mannitol, RNA-based drug

Abstract

The spectral characteristics of the complexation between the RNA and ligands such as mannitol, sorbitol, and lactose, by using the methods of FTIR and FT-Raman spectroscopy and the mathematical model MCR-ALS, are studied. The complexation optimal ratios are determined in the RNA ligand system. In the RNA: mannitol mixture, the ratio is 3 : 1 with complexation about 100 %. In the case of a RNA: lactose mixture, the complexation decreases up to 30 %, and for a RNA: sorbitol mixture, up to 25 %. The results are in a good agreement with the antiviral activity of drugs on the cell culture. The optimal ratio and the better complexation in a RNA: mannitol mixture correspond to the best biological activity of this system. As a result of the replacement of mannitol by lactose in the RNA-ligand system, the biological activity of the preparation changes from antiviral preparation to immunomodulating and anti-inflammatory ones.

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Published

23.12.2024

How to Cite

Vivcharyk, M., Ilchenko, O., Levchenko, S., & Tkachuk, Z. (2024). Complexation of RNA with mannitol, its spectral characteristics and biological activity . Reports of the National Academy of Sciences of Ukraine, (10), 78–83. https://doi.org/10.15407/dopovidi2016.10.078

Issue

No. 10 (2016): Reports of the National Academy of Sciences of Ukraine

Section

Biology

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Copyright (c) 2024 Reports of the National Academy of Sciences of Ukraine

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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