Estimation of the interaction of oxidized graphene with rat's erythrocyte membranes and blood plasma proteins by the method of spin probes

Authors

  • N.T. Kartel Chuiko Institute of Surface Chemistry of the NAS of Ukraine, Kiev
  • L.V. Ivanov Chuiko Institute of Surface Chemistry of the NAS of Ukraine, Kiev
  • V.A. Karachevtsev B. Verkin Institute for Low Temperature Physics and Engineering of the NAS of Ukraine, Kharkiv
  • A.N. Lyapunov Institute for Single Crystals of the NAS of Ukraine, Kharkiv
  • O.A. Nardid Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
  • Ya.O. Cherkashina Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
  • V.S. Leontiev B. Verkin Institute for Low Temperature Physics and Engineering of the NAS of Ukraine, Kharkiv
  • А.Yu. Ivanov B. Verkin Institute for Low Temperature Physics and Engineering of the NAS of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/dopovidi2017.08.071

Keywords:

carbon nanotubes, cytotoxicity of oxidized grapheme, microviscosity of erythrocyte membranes, serum albumin, spin probe method

Abstract

The effect of oxidized graphene on the microviscosity of erythrocyte membranes, as well as the viscosity of the water-protein plasma phase, is studied by the spin probe method. The introduction of oxidized graphene into a suspension of erythrocytes did not lead to significant changes in the microviscosity of their membranes. After four hours of incubation, an insignificant increase in the microviscosity is observed, apparently due to the sorption of oxidized graphene on membranes. The obtained results indicate the stability of the structure of erythrocyte membranes upon the interaction with oxidized graphene, which can be considered a sufficiently cytocompatible material. The introduction of oxidized graphene into plasma leads to a gradual increase in the viscosity of the water-protein phase from the macromolecules of serum albumin and other plasma proteins within the first 24 hours. The obtained data confirm the insignificant cytotoxicity of oxidized graphene in comparison with carbon nanotubes, which are able to significantly increase the membrane microviscosity of erythrocyte and break their integrity.

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References

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Published

15.09.2024

How to Cite

Kartel, N., Ivanov, L., Karachevtsev, V., Lyapunov, A., Nardid, O., Cherkashina, Y., Leontiev, V., & Ivanov А. (2024). Estimation of the interaction of oxidized graphene with rat’s erythrocyte membranes and blood plasma proteins by the method of spin probes . Reports of the National Academy of Sciences of Ukraine, (8), 71–79. https://doi.org/10.15407/dopovidi2017.08.071

Issue

No. 8 (2017): Reports of the National Academy of Sciences of Ukraine

Section

Chemistry

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