Influence of human placenta extracts to erythrocytes' thermohemolysis

Authors

  • O.M. Bobrova nstitute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
  • Yu.S. Govorova nstitute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
  • O.A. Nardid Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
  • S.V. Repina Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
  • S.V. Narozhnyi Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv
  • S.V. Pokryshko Institute for Problems of Cryobiology and Cryomedicine of the NAS of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/dopovidi2018.07.082

Keywords:

antiinflammatory efficacy, erythrocytes' thermohemolysis, fraction, freezing, placenta extract

Abstract

The influence of the freezing of human placenta extracts on their ability to reduce the thermohemolysis of erythrocytes has been investigated. It has been shown that the freezing of placenta extracts down to −2 and −19 °C and the following heating do not lead to a significant change in their effectiveness to stabilize the erythrocyte membranes at temperature effects. The placenta extract fractions, namely less than 4 kDa and 12—20 kDa, most effectively reducing the level of erythrocytes' thermohemolysis, have been identified. The percentages of inhibition of the thermogemolysis for them are (31.7 ± 3.3) % and (31.5 ± 3.2) %, respectively.

Downloads

Download data is not yet available.

References

Grischenko, V. I. & Yurchenko, T. N. (Eds.). (2011). Placenta: cryopreservation, structure, properties, prospects of clinical use. Kharkiv (in Russian).

Tiwary, S. K., Shukla, D., Tripathi, A.K., Agrawal, S., Singh, M. K. & Shukla, V. K. (2006). Effect of placentalextract gel and cream on healing wounds. J. Wound Care, 15, No. 7, pp. 325-328. doi: https://doi.org/10.12968/jowc.2006.15.7.26937

De, D., Chakraborty, P. D. & Bhattacharyya, D. (2011). Regulation of trypsin activity by peptide fraction of aqueous extract of human placenta used as wound healer. J. Cell Physiol., 226, No. 8, pp. 2033-2040. doi: https://doi.org/10.1002/jcp.22535

Bhadoriya, S. S., Mishra, V., Raut, S., Ganeshpurkar, A. & Jain, S. K. (2012). Anti-Inflammatory and antinociceptive activities of a hydroethanolic extract of tamarindus indica leaves. Sci. Pharm., 80, No. 3, pp. 685-700. doi: https://doi.org/10.3797/scipharm.1110-09

Okoli, C. O., Akah, P. A, Onuoha, N. J., Okoye, Th. C., Nwoye, A. C. & Nworu, Ch. S. (2008). Acanthus montanus: An experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles. BMC Compl. Alternative Med., 8: 27. doi: https://doi.org/10.1186/1472-6882-8-2

Bougandoura, A., D'Abrosca, B., Ameddah, S., Scognamiglio, M., Mekkiou, R., Fiorentino, A., Benayache, S. & Benayache, F. (2016). Chemical constituents and in vitro anti-inflammatory activity of Cistanche violacea Desf. (Orobanchaceae) extract. Fitoterapia, 109, No. 2, pp. 248-253. doi: https://doi.org/10.1016/j.fitote.2016.01.010

Banerjee, K. K., Bishayee, A. & Chatterjee, M. (1994). Role of human placental extract on succinic dehydrogenase activity in carrageenin-induced edema in rats in vivo and its effect on erythrocyte lysis, platelet aggregation and trypsin activity in vitro. Indian J. Physiol. Pharmacol., 38, No. 2, pp. 121-124. PMID: 8063356

Banerjee, K. K., Bishayee, A. & Chatterjee, M. (1992). Anti-inflammatory effect of human placental extract: a biochemical mechanistic approach. Riv. Eur. Sci. Med. Farmacol., 14, No. 6, pp. 361-366. PMID: 1308603

Sharma, K. & Bhattacharyya, D. (2015). Immunoglobulin isotype isolated from human placental extract does not interfere in complement-mediated bacterial opsonization within the wound milieu. FEBS Open Bio, 5, pp. 369-377. doi: https://doi.org/10.1016/j.fob.2015.04.007

Anosike, Ch. A., Obidoa, On. & Ezeanyika, L. (2012). US Membrane stabilization as a mechanism of the antiinflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU, 20: 76. doi: https://doi.org/10.1186/2008-2231-20-76

Ivanov, I. T., Brähler, M., Georgieva, R. & Bäumler, H. (2007). Role of membrane proteins in thermal damage and necrosis of red blood cells. Thermochim. Acta, 456, pp. 7-12. doi: https://doi.org/10.1016/j.tca.2007.01.020

Published

15.05.2024

How to Cite

Bobrova, O., Govorova, Y., Nardid, O., Repina, S., Narozhnyi, S., & Pokryshko, S. (2024). Influence of human placenta extracts to erythrocytes’ thermohemolysis . Reports of the National Academy of Sciences of Ukraine, (7), 82–87. https://doi.org/10.15407/dopovidi2018.07.082