Genomics and virion DNA structure of temperate erwiniophages 49 and 59
DOI:
https://doi.org/10.15407/dopovidi2017.06.082Keywords:
bacteriophages 49 and 59, bioinformatic analysis, circular and discrete permutation, headful packaging mechanism, restriction analysisAbstract
A comparative study of virion DNAs of phages 49 and 59 by the standard restriction and bioinformatic analyses is conducted. It has been shown that the genomes of both bacteriophages are cyclically permuted, but phage 59 is characterized by continuous permutation of the genome, while phage 49 has discrete permutation. Virion DNA of phage 49 has an abnormal cleavage site for SmaI restriction enzyme that, unlike the rest SmaIsites, is not completely hydrolyzed.
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