Effects of COVID-19 and diabetes mellitus on AMPKα1 and IRS-1 amount in the blood plasma of patients

M.D.  Tronko; V.V.  Pushkarev; L.K.  Sokolova; S.A.  Cherviakova; Y.B.  Belchina; O.I.  Kovzun; B.B.  Guda; V.M. Pushkarev

doi:10.15407/dopovidi2022.03.087

Authors

M.D. Tronko V.P. Komisarenko Institute of Endocrinology and Metabolism https://orcid.org/0000-0001-7421-0981
V.V. Pushkarev V.P. Komisarenko Institute of Endocrinology and Metabolism https://orcid.org/0000-0001-5940-5510
L.K. Sokolova V.P. Komisarenko Institute of Endocrinology and Metabolism https://orcid.org/0000-0003-0011-0106
S.A. Cherviakova V.P. Komisarenko Institute of Endocrinology and Metabolism https://orcid.org/0000-0002-6917-5736
Y.B. Belchina V.P. Komisarenko Institute of Endocrinology and Metabolism https://orcid.org/0000-0002-4289-8977
O.I. Kovzun V.P. Komisarenko Institute of Endocrinology and Metabolism https://orcid.org/0000-0001-8164-7671
B.B. Guda V.P. Komisarenko Institute of Endocrinology and Metabolism https://orcid.org/0000-0002-9181-0679
V.M. Pushkarev V.P. Komisarenko Institute of Endocrinology and Metabolism https://orcid.org/0000-0003-0347-7771

DOI:

https://doi.org/10.15407/dopovidi2022.03.087

Keywords:

5′AMP-activated protein kinase α1, insulin receptor substrate-1, COVID-19, diabetes mellitus

Abstract

The aim of the study was to determine the levels of AMPKα1 and IRS-1 in the blood of patients with diabetes mellitus and COVID-19. AMPKα1 and IRS-1 were determined using enzyme-linked immunosorbent assay (ELISA) (Elabscience, USA). AMPK controls the energy balance of the cell, stimulates catabolic processes – absorption of glucose, fatty acids, and their conversion by mitochondrial oxidation and glycolysis. With type 2 diabetes and obesity, its activity decreases, and the activity of protein kinases mTORC1/p70S6K increases, leading to phosphorylation of insulin receptor substrate-1 (IRS) and insulin resistance. The level of AMPKα1 in the blood of diabetic patients was significantly higher than in the blood of healthy people. The amount of AMPKα1 in the blood of people recovered from COVID-19 demonstrated the further growth of AMPKα1. The level of AMPKα1 was much higher in the blood of patients with DM during a COVID-19 disease. IRS-1 amounts in the blood plasma of patients with diabetes was higher than normal values. The level of IRS-1 in the blood plasma of patients with COVID-19 was much higher than in the blood of healthy people and patients with diabetes. The level of IRS-1 in the blood plasma may be one of the promising markers of COVID-19.

Downloads

Download data is not yet available.

References

Ruderman, N. B., Carling, D., Prentki, M. & Cacicedo, J. M. (2013). AMPK, insulin resistance, and the metabolic syndrome. J. Clin. Invest., 123, No. 7, pp. 2764-2772. https://doi.org/10.1172/JCI67227

https://doi.org/10.1172/JCI67227

Xiao, B., Sanders, M. J., Underwood, E., Heath, R., Mayer, F. V., Carmena, D., Jing, C., Walker, P. A., Eccleston, J. F., Haire, L. F., Saiu, P., Howell, S. A., Aasland, R., Martin, S. R., Carling, D. & Gamblin, S. J. (2011). Structure of mammalian AMPK and its regulation by ADP. Nature., 472, No. 7342, pp. 230-233. https://doi.org/10.1038/nature09932

https://doi.org/10.1038/nature09932

Carling, D., Mayer, F. V., Sanders, M. J. & Gamblin, S. J. (2011). AMP-activated protein kinase: Nature's energy sensor. Nat. Chem. Biol., 7, pp. 512-518. https://doi.org/10.1038/nchembio.610

https://doi.org/10.1038/nchembio.610

Racioppi, L. & Means, A. R. (2012). Calcium/calmodulin-dependent protein kinase kinase 2: roles in signaling and pathophysiology. J. Biol. Chem., 287, No. 38, pp. 31658-31665. https://doi.org/10.1074/jbc.R112.356485

https://doi.org/10.1074/jbc.R112.356485

Jeong, K. J., Kim, G. W. & Chung, S. H. (2014). AMP-activated protein kinase: An emerging target for ginseng. J. Ginseng Res., 38, No. 2, pp. 83-88. https://doi.org/10.1016/j.jgr.2013.11.014

https://doi.org/10.1016/j.jgr.2013.11.014

Saha, A. K., Xu, X. J., Balon, T. W., Brandon, A., Kraegen, E. W. & Ruderman, N. B. (2011). Insulin resistance due to nutrient excess. Is it a consequence of AMPK downregulation? Cell Cycle, 10, No. 20, pp. 3447-3451. https://doi.org/10.4161/cc.10.20.17886

https://doi.org/10.4161/cc.10.20.17886

Filippatos, T. D., Liberopoulos, E. N. & Elisaf, M. S. (2015). Dapagliflozin in patients with type 2 diabetes mellitus. Ther. Adv. Endocrinol. Metab., 6, No. 1, pp. 29-41. https://doi.org/10.1177/2042018814558243

https://doi.org/10.1177/2042018814558243

Tronko, N. D., Kovzun, E. I., Pushkarev, V. V., Sokolova, L. K. & Pushkarev, V. M. (2018). Reception and intracellular mechanisms of insulin action (рart 1). Endokrynologia, 23, No. 3, pp. 269-280 (in Russian).

https://doi.org/10.31793/1680-1466.2018.23-4.341

Porter, H. A., Perry, A., Kingsley, C., Tran, N. L. & Keegan, A. D. (2013). IRS1 is highly expressed in localized breast tumors and regulates the sensitivity of breast cancer cells to chemotherapy, while IRS2 is highly expressed in invasive breast tumors. Cancer Lett., 338, No. 2, pp. 239-248. https://doi.org/10.1016/j.canlet.2013.03.030

https://doi.org/10.1016/j.canlet.2013.03.030

Sun, X., Chen, Y., Tan, J. & Qi, X. (2018). Serum IRS-1 acts as a novel biomarker for diagnosis in patients with nasopharyngeal carcinoma. Int. J. Clin. Exp. Pathol., 11, No. 7, pp. 3685-3690.

Chan, S.-H., Kikkawa, U., Matsuzaki, H., Chen, J.-H. & Chang, W.-C. (2012). Insulin receptor substrate-1 prevents autophagy-dependent cell death caused by oxidative stress in mouse NIH/3T3 cells. J. Biomed. Sci., 19, Art. 64. https://doi.org/10.1186/1423-0127-19-64

https://doi.org/10.1186/1423-0127-19-64

Wang, X., Zimmermann, H. R., Lockhart, S. N., Craft, S. & Ma, T. (2020). Decreased levels of blood AMPKα1 but not AMPKα2 isoform in patients with mild cognitive impairment and Alzheimer's disease: A pilot study. J. Alzheimer's Dis., 76, No. 1, pp. 217-224. https://doi.org/10.3233/JAD-191189

https://doi.org/10.3233/JAD-191189

Zhang, M., Zhu, H., Ding, Y., Liu, Z., Cai, Z. & Zou, M.-H. (2017). AMP-activated protein kinase α1 promotes atherogenesis by increasing monocyte-to-macrophage differentiation. J. Biol. Chem., 292, No. 19, pp. 7888-7903. https://doi.org/10.1074/jbc.M117.779447

https://doi.org/10.1074/jbc.M117.779447

Hakuno, F., Fukushima, T., Yoneyama, Y., Kamei, H., Ozoe, A., Yoshihara, H., Yamanaka, D., Shibano, T., Sone-Yonezawa, M., Yu, B.-C., Chida, K. & Takahashi, S.-I. (2015). The novel functions of high-mo le cu larmass complexes containing insulin receptor substrates in mediation and modulation of insulin-like acti viISSN ties: emerging concept of diverse functions by IRS-associated proteins. Front. Endocrinol., 6, Art. 73. https://doi.org/10.3389/fendo.2015.00073

https://doi.org/10.3389/fendo.2015.00073

Reuveni, H., Flashner-Abramson, E., Steiner, L., Makedonski, K., Song, R., Shir, A., Herlyn, M., Bar-Eli, M. & Levitzki, A. (2013). Therapeutic destruction of insulin receptor substrates for cancer treatment. Cancer Res., 73, No. 14, pp. 4383-4394. https://doi.org/10.1158/0008-5472.CAN-12-3385

https://doi.org/10.1158/0008-5472.CAN-12-3385

Effects of COVID-19 and diabetes mellitus on AMPKα1 and IRS-1 amount in the blood plasma of patients

Authors

DOI:

Keywords:

Abstract

Downloads

References

Downloads

Published

How to Cite

Issue

Section

License

Most read articles by the same author(s)

Language

1

ISSN

Archives 2014-2020

Archive 2014-2020