Adaptor protein Ruk/CIN85 modulates manifestation of cancer stem cells (CSCs) features in mouse breast adenocarcinoma 4T1 cells

Authors

  • I.R. Horak Palladin Institute of Biochemistry of the NAS of Ukraine, Kiev
  • G.V. Pasichnyk Palladin Institute of Biochemistry of the NAS of Ukraine, Kiev
  • D.S. Gerashchenko Palladin Institute of Biochemistry of the NAS of Ukraine, Kiev
  • L. Knopfova Masaryk University, Brno, Czech Republic
  • L. Borsig Zurich University, Switzerland
  • L.B. Drobot Palladin Institute of Biochemistry of the NAS of Ukraine, Kiev

DOI:

https://doi.org/10.15407/dopovidi2018.12.101

Keywords:

adaptor protein Ruk/CIN85, breast adenocarcinoma, cancer stem cells, reprogramming, spheroids

Abstract

Using mouse breast adenocarcinoma 4T1 cells with stable overexpression or knockdown of adaptor protein Ruk/CIN85, its contribution to the manifestation of cancer stem cells features was estimated. It was demonstrated that the expression levels of reprogramming factors Klf4, Pou5f1/Oct4 and Nanog, as well as CSCs surface markers Cd44 and Cd24, depended on Ruk/CIN85 expression level: increased in the case of up-regulation and decreased in the case of down-regulation. It was found that the transcriptome patterns in 4T1 subclones with different expression levels of Ruk/CIN85 correlated with their ability to form mammospheres.

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Published

20.05.2024

How to Cite

Horak, I., Pasichnyk, G., Gerashchenko, D., Knopfova, L., Borsig, L., & Drobot, L. (2024). Adaptor protein Ruk/CIN85 modulates manifestation of cancer stem cells (CSCs) features in mouse breast adenocarcinoma 4T1 cells . Reports of the National Academy of Sciences of Ukraine, (12), 101–109. https://doi.org/10.15407/dopovidi2018.12.101

Issue

No. 12 (2018): Reports of the National Academy of Sciences of Ukraine

Section

Biochemistry

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