ROLE OF POLYAMINES IN FORMATION OF PLANT RESISTANCE TO SALT STRESS AND ZINC EXCESS IN MUTANTS OF PHOSPHOLIPASE D GENES
DOI:
https://doi.org/10.15407/dopovidi2025.05.019Keywords:
polyamines, phospholipase D, isoenzymes, phosphatidic acid, salt stress, zinc excessAbstract
Polyamines, which exhibit hormonal activity, play a key role in plant growth, development and stress response. The interaction of specific components of lipid signaling, in particular phospholipase D (PLD) and phosphatidic acid (PA), with polyamines in plants was investigated earlier. At the same time, the role of specific PLD isoen- zymes in plant responses to stress modulated by polyamines has remained unexplored. In order to clarify the role of PLD isoenzymes in the formation of plant tolerance to salt stress and zinc excess by polyamines, an analysis of morpho-physiological parameters and biochemical markers of stress resistance was carried out in Arabidopsis thaliana plants mutated in genes encoding various PLD isoenzymes. The results indicate that specific PLD iso- forms, in particular PLDγ1, PLDγ3 and PLDδ, play a key role in the action of polyamines in the formation of the resistance of Arabidopsis thaliana plants to stresses. This is evidenced by the absence of the restoration of morpho- logical and physiological parameters of plants, modulation of membrane stability and balance of active oxygen species in PLDγ1, PLDγ3, and PLDδ knockout mutants in response to polyamine action. Therefore, the isoen- zymes PLDγ1, PLDγ3, and PLDδ play a role in the action of polyamines in the development of resistance to salt stress and zinc excess.
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