Intron length polymorphism of actin genes as an efficient tool for the genetic profiling of selected cereals from the grass (Poaceae L.) family
DOI:
https://doi.org/10.15407/dopovidi2019.02.078Keywords:
actin genes, genetic profiling, intron length polymorphism, Poaceae LAbstract
The genetic profiling of wheat, barley and rice varieties was carried out by evaluating the intron length polymorphism of the actin genes for the first time. The differences were found between the wheat and barley varieties, and a sample of rice varieties was characterized as genetically monomorphic. In general, the possibility of using the assessment of the intron length polymorphism of the actin genes for the molecular genetic analysis of the grass family (Poaceae L.) has been proved.
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Jaikishan, I., Rajendrakumar, P., Madhusudhana. R., Elangovan, M. & Patil, J.V. (2015). Development and utility of PCRbased intron polymorphism markers in sorghum [Sorghum bicolor (L.) Moench]. J. Crop Sci. Biotechnol., 18, pp. 309-318. doi: https://doi.org/10.1007/s12892-015-0015-y
Muthamilarasan, M., Venkata Suresh, B., Pandey, G., Kumari, K., Parida, S.K. & Prasad, M. (2013). Development of 5123 intronlength polymorphic markers for largescale genotyping applications in foxtail millet. DNA Res., 21, No. 1, pp. 41-52. doi: https://doi.org/10.1093/dnares/dst039
Wang, X., Zhao, X., Zhu. J. & Wu, W. (2005). Genomewide investigation of intron length polymorphisms and their potential as molecular markers in rice (Oryza sativa L.). DNA Res., 12, No. 6, pp. 417-427. doi: https://doi.org/10.1093/dnares/dsi019
Yang, L., Jin, G., Zhao, X., Zheng, Y., Xu, Z. & Wu, W. (2007). PIP: a database of potential intron polymorphism markers. Bioinformatics, 23, No. 16, pp. 2174-2177. doi: https://doi.org/10.1093/bioinformatics/btm296
Huang, L., Cao, H., Yang, L., Yu, Y. & Wang, Y. (2013). Largescale development of PIP and SSR markers and their complementary applied in Nicotiana. Russ. J. Genet., 49, pp. 827-838. doi: https://doi.org/10.1134/S1022795413070077
Xia, X., Luan, L. L., Qin, G., Yu, L. F., Wang, Z.W., Dong, W.C., Song, Y., Qiao, Y., Zhang, X. S., Sang, Y. L. & Yang L. (2017). Genomewide analysis of SSR and ILP markers in trees: diversity profiling, alternate distribution, and applications in duplication. Sci. Rept., 7, No. 1, pp. 1-10. doi: https://doi.org/10.1038/s41598017172036
Rabokon, A.N., Pirko, Ya.V., Demkovych, A.Ye. & Blume, Ya.B. (2018). Comparative analysis of the efficiency of intronlength polymorphism of btubulin genes and microsatellite loci for flax varieties genotyping. Cytol. Genet., 52, No. 1, pp. 3-15. doi: https://doi.org/10.3103/S0095452718010115
Postovoitova, A.S., Yotka, O.Yu., Pirko, Ya.V. & Blume, Ya.B. (2018). Molecular genetic evaluation of Ukrainian flax cultivars homogeneity based on intron length polymorphism of actin genes and microsatellite loci. Cytol. Genet., 52, No. 6, pp. 448-460. doi: https://doi.org/10.3103/S0095452718060099
Bardini, M., Lee, D., Donini, P., Mariani, A., Giani, S., Toschi, M., Lowe, C. & Breviario, D. (2004). Tubulinbased polymorphism (TBP): a new tool, based on functionally relevant sequences, to assess genetic diversity in plant species. Genome, 47, No. 2, pp. 281-291. doi: https://doi.org/10.1139/g03-132
Rabokon, A.N., Demkovich, A.E., Pirko, Ya.V. & Blume, Ya.B. (2015). Studing of btubulin gene intron length polymorphizm of Triticum aestivum L. and Hordeum vulgare L. varieties. Factors Exp. Evol. Organisms, 17, pp. 82-86 (in Ukrainian).
Rabokon, A., Demkovich, A., Sozinov, A., Kozub, N., Pirko, Ya. & Blume, Ya. (2017). Intron length poly morphism of btubulin genes of Aegilops biuncialis Vis. Cell Biol. Int. doi: https://doi.org/10.1002/cbin.10886
Rabokon, A.N., Demkovych, A.Ye., Pirko, Ya.V. & Blume, Ya.B. (2016). Studying of intron length polymorphism of btubulin genes in plants of genus Linum L. Factors Exp. Evol. Organisms, 19, pp. 43-46 (in Ukrainian).
Postovoitova, A.S., Pirko, Ya.V. & Blume, Ya.B. (2018). Polymorphism of actin gene introns as an instrument for genotyping of the representatives from Solanaceae family. Sci. Bull. NUBiP, 287, pp. 70-78 (in Ukrainian).
Sambrook, J.F. & Russell, D.W. (2001). Molecular ñloning: a laboratory manual. (Vol. 3). New York: Cold Spring Harbor Laboratory Press. Received 21.01.2019.
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