Antioxidant properties of plant extracts for biodiesel stabilization
DOI:
https://doi.org/10.15407/dopovidi2021.02.091Keywords:
plant extracts, phenolic compounds, antioxidant properties, biodiesel stabilityAbstract
Using two different extraction procedures, eight ethanol plants extracts are obtained from the leaves of Magnolia X soulangeana Soul.-Bod., Magnolia kobus, and two samples of Camellia japonica L. The composition and antioxidant properties of the extracts are studied by means of high performance liquid chromatography, the Folin— Ciocalteu method, and DPPH test. Hydroxycinnamic acids and quercetin glicosides are found to be the main constituents of Magnolia extracts, while hydroxybenzoic acids and catechin derivatives prevailed in Camelia extracts. The composition of the extracts was also affected by extraction procedures; in general, the extracts obtained at 60 °C under ultrasonic treatment contained phenolic compounds of a higher quantity than the extracts prepared by boiling the leaves in 70 % ethanol at ~85 °C; the overall amount of phenolic compounds in the extracts was in a range of 50-150 mg/l. In spite of significant distinctions in the content of phenols, all the extracts were found to possess a very high antioxidant activity in both Folin—Ciocalteu and DPPH assays. The extracts were found to have the total phenolic index of 1.5-7.5. During 30 min of the reaction, seven of eight extracts inhibited more than 50 % of DPPH radicals under standard test conditions, even being diluted by 10 times. The extract of Camellia japonica L. with the highest antioxidant ability was also tested as an additive to stabilize the biodiesel against oxidation. The stability of biodiesel prepared from Camelina sativa (L.) Crantz was studied according to accelerated procedure at 43 °C for four weeks, with the changes in the acid value of the samples being the criteria of fuel oxidation. The preliminary results showed that Camellia extract may be a promising stabilizing additive to reduce the biodiesel oxidation.
Downloads
References
Pandey, K. B. & Rizvi, S. I. (2009). Plant polyphenols as dietary antioxidants in human health and disease.
Oxid. Med. Cell. Longev., 2, No. 5, pp. 270-278. https://doi.org/10.4161/oxim.2.5.9498
Stavinskaya, O., Laguta, I., Fesenko, T. & Krumova, M. (2019). Effect of temperature on green synthesis of
silver nanoparticles using Vitex agnus-castus extract. Chem. J. Mold., 14, No. 2, pp. 1857-1727. https://doi.
org/10.19261/cjm.2019.636
Varatharajan, K. & Pushparani, D. S. (2018). Screening of antioxidant additives for biodiesel fuels. Renew.
Sustain. Energу Rev., 82, No. 3, pp. 2017-2028. https://doi.org/10.1016/j.rser.2017.07.020
Laguta, I. V., Stavinskaya, О. N., Dzyuba, О. I. & Ivannikov, R. V. (2015). Analysis of antioxidant properties
of plants extracts. Dopov. Nac. akad. nauk. Ukr., No. 5, pp. 130-137 (in Russian). https://doi.org/10.15407/
dopovidi2015.05.130
Park, C. H., Park, S.-Y., Lee, S. Y., Kim, J. K. & Park, S. U. (2018). Analysis of metabolites in white flowers of
Magnolia denudata Desr. and violet flowers of Magnolia liliiflora Desr. Molecules, 23, No. 7, pp. 1558-1574.
https://doi.org/10.3390/molecules23071558
Yoon, I.-S., Park, D.-H., Kim, J.-E., Yoo, J.-C., Bae, M.-S., Oh, D.-S., Shim, J.-H., Choi, C.-Y., An, K.-W., Kim,
E.-I., Kim, G.-Y. & Cho, S.-S. (2017). Identification of the biologically active constituents of Camellia japonica
leaf and anti-hyperuricemic effect in vitro and in vivo. Int. J. Mol. Med., 39, No. 6, pp. 1613-1620. https://doi.
org/10.3892/ijmm.2017.2973
Alonso, A. M., Domínguez, C., Guillén, D. & Barroso, C.G. (2002). Determination of antioxidant power of
red and white wines by a new electrochemical method and its correlation with polyphenolic content. J. Agric.
Food Chem., 50, No. 11, pp. 3112-3115. https://doi.org/10.1021/jf0116101
Brand-Williams, W., Cuvelier, M.E. & Berset, C. (1995). Use of a free radical method to evaluate antioxidant
activity. LWT, 28, No. 1, pp. 25-30. https://doi.org/10.1016/S0023-6438(95)80008-5
Westbrook, S. R. (2005). An evaluation and comparison of test methods to measure the oxidation stability of
neat biodiesel. San Antonio, Texas: Southwest Research Institute.
Yakovlieva, A. V., Boichenko, S. V., Hudz, A. V. & Zubenko, S. O. (2020). Physical-chemical properties of
biodiesel fuels based on camelina oil ethyl esters. Catalysis and petrochemistry, No. 29, pp. 24-31 (in
Ukrainian). https://doi.org/10.15407/kataliz2020.29.027
DSTU 4350:2004 Oils. Methods for determining the acid number (ISO 660:1996, NEQ). Kyiv, 2005.
Laguta, I.V., Stavinskaya, О.N., Oranskaya, E.I. & Chernyavskaya, T.V. (2009). Interaction of ascorbic acid
with highly dispersed silica. Dopov. Nac. akad. nauk Ukr., No. 12, pp. 152-157 (in Russian).
Zenkevich, I.G., Eshchenko, A.Yu., Makarova, S.V., Vitenberg, A.G., Dobryakov, Y.G. & Utsal, V.A. (2007).
Identification of the products of oxidation of quercetin by air oxygen at ambient temperature. Molecules, 12,
No. 3, pp. 654-672. https://doi.org/10.3390/12030654
Volf, I., Ignat, I., Neamtu, M. & Popa, V.I. (2014). Thermal stability, antioxidant activity, and photo-oxidation
of natural polyphenols. Chem. Pap., 68, No. 1, pp. 121—129. https://doi.org/10.2478/s11696-013-0417-6
McCormick, R.L. & Westbrook, S.R. (2010). Storage stability of biodiesel and biodiesel blends. Energy Fuels,
, No. 1, pp. 690—698. https://doi.org/10.1021/ef900878u
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Reports of the National Academy of Sciences of Ukraine
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
