An inhibitor of Ca2+-dependent protein kinases, trifluoperazine, increases the efficiency of agrobacterium-mediated transformation of tobacco
DOI:
https://doi.org/10.15407/dopovidi2014.11.165Keywords:
agrobacterium-mediated transformation, efficiency, tobaccoAbstract
The influence of different concentrations of the inhibitor of Ca2+-calmodulin-dependent protein kinase, trifluoperazine, on the efficacy of the agrobacterium-mediated transformation of Nicotiana tabacum leaf explants is investigated. It is established that the addition of 10 and 25 μM trifluoperazine into the medium for the co-cultivation with agrobacterium led to increasing the transformation rate up to 98%, that higher by 25% as compared to control. We conducted a series of experiments on the selection of conditions for the agrobacterium-mediated transformation by AGL1 strain that contained plasmid pGH217 and determined the most efficient methodology of genetic transformation.
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