Gametophyte morphogenesis of a fern Polystichum aculeatum L. Roth of Ukraine's flora in culture in vitro

Authors

  • L.M. Babenko M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev
  • K.O. Romanenko M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev
  • M.M. Shcherbatiuk M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev
  • O.V. Vasheka Institute of Biology and Medicine, Taras Shevchenko National University of Kiev
  • P.O. Romanenko Institute of Biology and Medicine, Taras Shevchenko National University of Kiev
  • V.A. Negretsky M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev
  • I.V. Kosakivska M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev

DOI:

https://doi.org/10.15407/dopovidi2017.10.101

Keywords:

gametophyte, Polystichum aculeatum, prothallus, protonema, thallus

Abstract

The spore morphology and the germination pattern, as well as peculiarities of the gametophyte development of a wild fern Polystichum aculeatum (L.) Roth. in culture in vitro are studied. The spore germination is found to be dependent on the duration of their storage. 80—95 % of freshly-picked spores have germinated in culture in vitro. After the 4—6-month storage under lab conditions, the germinating ability ranged within 68—95 %; after 1.5 months, it did not exceed 20 %. The time limits of four stages in gametophyte morphogenesis are singled out and established. The spore germination and the protonema formation in P. Aculeatum are shown to occur according to the Vittaria-pattern, prothallium development — according to the Aspidium-pattern.

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References

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Published

21.09.2024

How to Cite

Babenko, L., Romanenko, K., Shcherbatiuk, M., Vasheka, O., Romanenko, P., Negretsky, V., & Kosakivska, I. (2024). Gametophyte morphogenesis of a fern Polystichum aculeatum L. Roth of Ukraine’s flora in culture in vitro . Reports of the National Academy of Sciences of Ukraine, (10), 101–107. https://doi.org/10.15407/dopovidi2017.10.101

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