Phytohormonal regulation of the development of Dryopteris filix-mas (L.) Schott gametophyte in vitro culture

Authors

  • K.O. Romanenko M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev
  • L.M. Babenko M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev
  • O.V. Vasheka Institute of Biology and Medicine of Taras Shevchenko National University of Kiev
  • P.O. Romanenko Institute of Biology and Medicine of Taras Shevchenko National University of Kiev
  • I.V. Kosakivska M.G. Kholodny Institute of Botany of the NAS of Ukraine, Kiev

DOI:

https://doi.org/10.15407/dopovidi2018.11.096

Keywords:

6-benzylaminopurine, cytokinins, Dryopteris filix-mas, gametophyte, kinetin, N6-2-isopentenyladenine, prothallium, spores, thallus, zeatin

Abstract

Effects of exogenous cytokinin phytohormones such as kinetin, zeatin, 6-benzylaminopurine, and N6-2-isopentenyladenine on the gametophyte morphology and growth features of Dryopteris filix-mas (L.) Schott in vitro culture have been studied. It is established that, at the concentration of 10−5 M, all studied cytokinins inhibit the gametophyte growth, cause deformations and changes in the thallus size, and suppress the development of reproductive structures and sporophyte growth. Reduction of the hormone concentration to 10−8 M stimulates the gametophyte development, induces cell divisions, particularly in the apical zone, due to which some of thalli are deformed, promotes the production of rhizoids, affects the formation of antheridia and archegonia, and slows the sporophyte development.

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References

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Published

20.05.2024

How to Cite

Romanenko, K., Babenko, L., Vasheka, O., Romanenko, P., & Kosakivska, I. (2024). Phytohormonal regulation of the development of Dryopteris filix-mas (L.) Schott gametophyte in vitro culture . Reports of the National Academy of Sciences of Ukraine, (11), 96–105. https://doi.org/10.15407/dopovidi2018.11.096

Issue

No. 11 (2018): Reports of the National Academy of Sciences of Ukraine

Section

Ecology

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