Поліморфізм довжини інтронів генів γ-тубуліну як новий підхід до генотипування рослин

Ya.V. Pirko; D.D. Buy; A.S. Postovoitova; A.M. Rabokon; L.O. Kalafat; Ya.B. Blume

doi:10.15407/dopovidi2018.12.087

New ilp method based on γ-tubulin genes intron length polymorphism

Authors

Ya.V. Pirko Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kiev
D.D. Buy Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kiev
A.S. Postovoitova Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kiev
A.M. Rabokon Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kiev
L.O. Kalafat Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kiev
Ya.B. Blume Institute of Food Biotechnology and Genomics of the NAS of Ukraine, Kiev

DOI:

https://doi.org/10.15407/dopovidi2018.12.087

Keywords:

gene, intron, length of introns, molecular genetic markers, polymorphism, γ-tubulin

Abstract

A new marker system for the estimation of a genetic diversity based on the γ-tubulin genes intron length polymorphism is proposed. A pair of degenerate primers to γ-tubulin genes was developed based on the characteristics of their exon-intron structures. The simplicity and versatility of the marker system in the moleculargenetic analysis of plants are demonstrated on species and varieties of Arabidopsis thaliana, Linum usitatissimum, Solanum tuberosum, Oryza sativa, Triticum aestivum.

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Published

20.05.2024

How to Cite

Pirko, Y., Buy, D., Postovoitova, A., Rabokon, A., Kalafat, L., & Blume, Y. (2024). New ilp method based on γ-tubulin genes intron length polymorphism . Reports of the National Academy of Sciences of Ukraine, (12), 87–92. https://doi.org/10.15407/dopovidi2018.12.087