Cytogenetic effects in mixed culture of blood cells from patients with chronic lymphocytic leukemia with blood lymphocytes of healthy individuals
DOI:
https://doi.org/10.15407/dopovidi2020.07.086Keywords:
chromosome aberration, chronic lymphocytic leukemia, mixed culture of human peripheral blood lymphocytes, tumor-induced bystander effectAbstract
Using our own experimental model system to study the bystander response phenomenon in somatic human cells (co-culturing peripheral blood lymphocytes of donors of different gender, which makes it possible to distinguish cells-inductors and bystander cells by the presence or absence of male sex chromosome Y), we studied the features of the interaction between malignant and normal human cells. To carry out the research, two observation groups were formed. The comparison group included 7 conditionally healthy volunteers with an uncomplicated anamnesis (5 female, two male) who denied conscious contact with ionizing radiation and other mutagenic factors. The group of cancer patients included 7 persons (5 male, two female) with a diagnosis of primary B-cell chronic lymphocytic leukemia (CLL) before the start of specific therapy. It was found that co-cultivation of blood cells from CLL patients (cells-inductors) with intact peripheral blood lymphocytes of healthy individuals (bystander cells) leads to an increase in the frequency of chromosomal aberrations in normal cells (1.52 ± 0.30 and 3.31 ± 0.50 per 100 metaphases, respectively, p < 0.001), which confirms the development of the direct tumor-induced bystander effect (TIBE).
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