Intron length polymorphism of β-tubulin genes in microalgae

Authors

  • Ya.V. Pirko Institute of Food Biotechnology and Genomics
  • A.M. Rabokon Institute of Food Biotechnology and Genomics
  • A.S. Postovoitova Institute of Food Biotechnology and Genomics
  • Yu.O. Bilonozhko Institute of Food Biotechnology and Genomics
  • L.O. Kalafat Institute of Food Biotechnology and Genomics
  • O.V. Borisova M.G. Kholodny Institute of Botany
  • P.M. Tsarenko M.G. Kholodny Institute of Botany
  • Ya.B. Blume Institute of Food Biotechnology and Genomics

DOI:

https://doi.org/10.15407/dopovidi2019.01.093

Keywords:

introns, microalgae, molecular genetic markers β-tubulin genes

Abstract

The genetic profiling of 8 microalgae species (10 strains) from the collection IBASU-A of Institute of Botany M.G. Kholodny of the NAS of Ukraine was performed using the intron length polymorphism analysis of β-tu bulin genes. For the first time, the regularities of the differences between the DNA profiles of the microalgae taxonomic units on the generic level, namely Acutodesmus, Desmodesmus, Dunaliella, Tribonema, and Euglena were established. The possibility of using the method for estimating the intron length polymorphism of β-tubulin genes for the interspecies and intraspecific differentiations of microalgae in the polymorphism was demonstrated.

Downloads

References

Temraleeva, A.D., Mincheva, E.V., Sherbakov, D.Yu. & Pinsky, D.L. (2013). DNA-barcoding of green algae: a review. Аlgologia, 23, Nо. 4, pp. 396-418 (in Ukrainian). doi: https://doi.org/10.15407/alg23.04.396

Tsarenko, P., Borysova, O. & Blyume, Ya. (2011). Microalgas as bioenergetics object IBASU-A collection species — perspective producers of biomass as the source of raw stuff for biofuel. Visn. Nac. Acad. Nauk Ukr., Nо. 5, pp. 49-54 (in Ukrainian).

Chikkaswamy, B.K. & Paramanik, R.Ch. (2016). Molecular distinction of algae using molecular marker. Int. J. Curr. Microbiol. App. Sci., 5, No. 9, pp. 489-495 doi: https://doi.org/10.20546/ijcmas.2016.509.054

Matveeva, T.V., Pavlova, O.A., Bogomaz, D.I., Lutova, L.A. & Demkovich, A.E. (2011). Molecular markers for plant species identification and phylogenetics. Ecol. Genetics, 9, No. 1, pp. 32-43 (in Russian). doi: https://doi.org/10.17816/ecogen9132-43

Wongsawad, Ph. & Peerapornpisal, Yu. (2014). Molecular identification and phylogenetic relationship of green algae, Spirogyra ellipsospora (Chlorophyta) using ISSR and rbcL markers. Saudi J. Biol. Sci., 21, pp. 505-510. doi: https://doi.org/10.1016/j.sjbs.2014.01.003

Xia, X., Luan, L. L., Qin, G., Yu, L.F., Wang, Zh.W., Dong, W.Ch., Song, Y., Qiao, Y., Zhang, X.S., Sang, Y.L. & Yang, L. (2017). Genome-wide analysis of SSR and ILP markers in trees: diversity profiling, alternate distribution, and applications in duplication. Sci. Rep., 7, No. 1, 17902. doi: https://doi.org/10.1038/s41598-017-17203-6

Bardini, M., Lee, D., Donini, P., Mariani, A., Giani, S., Toschi, M., Lowe, C. & Breviario, D. (2004). Tubulinbased polymorphism (TBP): a new tool, based on functionally relevant sequences, to assess genetic diversity in plant species. Genome, 47, pp. 281-291. doi: https://doi.org/10.1139/g03-132

Rabokon, A.N., Pirko, Ya.V., Demkovych, A.Ye. & Blume, Ya.B. (2018). Comparative analysis of the efficiency of intron-length polymorphism of β-tubulin genes and microsatellite loci for flax varieties genotyping. Cytol. Genetics, 52, No. 1, pp. 1-10. doi: https://doi.org/10.3103/S0095452718010115

Sambrook, J. & Russell, D.W. (Eds.). (2001). Molecular сloning: a laboratory manual. (Vol. 2). Cold Spring Harbor: Cold Spring Laboratory Press.

Breviario, D., Baird, W.V., Sangoi, S., Hilu, K., Blumetti, P. & Gianì, S. (2007). High polymorphism and resolution in targeted fingerprinting with combined beta-tubulin introns. Mol. Breed., 20, pp. 249-259. doi: https://doi.org/10.1007/s11032-007-9087-9

Benbouza, H., Jean-Marie, J. & Jean-Pierre, B. (2006). Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. Biotechnol. Agron. Soc. Environ., 10, No. 2. pp. 77-81.

Lazar, I. (2010). GelAnalyzer.com. Retrieved from http://www.gelanalyzer.com/

Braglia, L., Manca, A., Mastromauro, F. & Breviario, D. (2010). cTBP: A successful intron length polymorphism (ILP)-based genotyping method targeted to well defined experimental needs. Diversity, 2, No. 4, pp. 572-585. doi: https://doi.org/10.3390/d2040572

Published

28.03.2024

How to Cite

Pirko, Y. ., Rabokon, A. ., Postovoitova, A. ., Bilonozhko, Y. ., Kalafat, L. ., Borisova, O. ., Tsarenko, P. ., & Blume, Y. . (2024). Intron length polymorphism of β-tubulin genes in microalgae . Reports of the National Academy of Sciences of Ukraine, (1), 93–99. https://doi.org/10.15407/dopovidi2019.01.093

Most read articles by the same author(s)

1 2 > >>