How do exogenous chemical factors affect the activity of the wheat genome? A study using the RNA/DNA ratio

Authors

  • O.I., Martynenko
  • T.K. Kyrylenko
  • A.V., Stepanyugin
  • D.P. Plodnik
  • D.M. Hovorun

DOI:

https://doi.org/10.15407/dopovidi2014.01.168

Keywords:

activity, chemical factors, DNA, genome, RNA, wheat

Abstract

This paper presents a study of the wheat plant response to the effect of the chemical factor (methisazon) at the level of genome reaction using the RNA/DNA ratio. We established a differential character of the wheat genome functioning in the process of growth using the RNA/DNA ratio and indicators of the transcriptional activity of ribosomal genes. We found the opposite directionality of changes in the RNA/DNA ratio and in the growth rate of leaves in all studied plants. This finding is supported by the presence of a negative correlation between these parameters. We suggest to use the RNA/DNA ratio for the quantitative evaluation of the impact level of chemical factors on the functional activity of the plant genome and the growth rate of plants.

Downloads

References

Hirayama T., Shinozaki K. Plant J., 2010, 61: 1041–52. https://doi.org/10.1111/j.1365-313X.2010.04124.x

Ideker T., Galitski T., Hood L. Annu. Rev. Genomics Hum. Genet., 2001, 2: 343–372. https://doi.org/10.1146/annurev.genom.2.1.343

Long T. A., Brady S. M., Benfey P. N. Annu. Rev. Cell Dev. Biol., 2008, 24: 81–103. https://doi.org/10.1146/annurev.cellbio.24.110707.175408

Rockman M. V., Kruglyak L. Nature Rev. Genet., 2006, 7: 862–872. https://doi.org/10.1038/nrg1964

Heinrich S., Valentin K., Frickenhaus S. et al. PLoS ONE, 2012, 7, Iss. 8: e44342. https://doi.org/10.1371/journal.pone.0044342

Quenelle D. C., Keith K. A., Kern E. R. Antiviral Res., 2006, 71, Iss. 1: 24–30. https://doi.org/10.1016/j.antiviral.2006.02.010

Levin H. G., Sharek K. M., Johnson K. M. et al. Adv. space res., 2000, 26, No. 2: 311–314. https://doi.org/10.1016/S0273-1177(99)00577-3

Martynenko O. I., Kyrylenko T. K., Alkhimova O. G. Dopov. Nac. akad. nauk Ukr., 2009, No. 2: 179–183 (in Russian).

Ausubel F. M., Brent R., Kingston R. E. et. al. (Eds.). Short protocols in molecular biology. 4th ed. New York: Wiley, 1999: 4–26.

Chicharo M. A., Chicharo L. Int. J. Mol. Sci., 2008, 9: 1453–1471. https://doi.org/10.3390/ijms9081453

Tishchenko E. N., Kurchy V. M., Petrov I. A. Ukr. biokhim. zhurn., 1999, 71, No. 5: 29–33.

Van Katz V. A., Thulke O. U., Conrath U. A. Plant Physiol., 1998, 117: 1333–1339. https://doi.org/10.1104/pp.117.4.1333

Kharina A., Zaets I., Ovcharenko L. et al. Sepsis, 2011, 4, No. 1: 73–78.

Umezawa T., Fujita M., Fujita Y. et al. Curr. opinion biotechnol., 2006, 17, No. 2: 113–122. https://doi.org/10.1016/j.copbio.2006.02.002

Published

24.03.2025

How to Cite

Martynenko, O., Kyrylenko, T., Stepanyugin, A., Plodnik, D., & Hovorun, D. (2025). How do exogenous chemical factors affect the activity of the wheat genome? A study using the RNA/DNA ratio . Reports of the National Academy of Sciences of Ukraine, (1), 168–173. https://doi.org/10.15407/dopovidi2014.01.168