Pseudomonas sp. strain 2303 as active phytopathogenic antagonist and its antibiotic characteristics
DOI:
https://doi.org/10.15407/dopovidi2014.10.161Keywords:
antagonist, antibiotic characteristics, phytopathogens, Pseudomonas sp. strain 2303Abstract
As a result of the screening among strains in the Ukrainian collection of microorganisms, Pseudomonas sp. strain 2303 with high antibacterial and antifungal activities was selected. The growth inhibition zone against phytopathogenic bacteria Pseudomonas syringae, Pectobacterium carotovorum, Xanthomonas campestris, Clavibacter michiganensis, and Agrobacterium tumefaciens was 18–39 mm; the inhibition index against micromycetes belonging to Fusarium, Bipolaris, Pythium, and Gaeumannomyces genera ranged from 39 to 51%. Pseudomonas sp. strain 2303 was also characterized by a high algicidal activity: cell-free liquid culture (1 : 5 dilution) repressed the growth of cyanobacteria Anabaena variabilis, Nostoc linckia, and Microcystis aeruginosa by 85–90%. HPLC-analysis of Pseudomonas sp. 2303 liquid culture revealed the antibiotic active substance — phenazine-1-carboxilic acid. Results of molecular-genetic analysis — amplification with primers to phzD gene — suggest that Pseudomonas sp. 2303 genome contains phenazine operon. Pseudomonas sp. 2303 can be considered as a perspective strain for the development of algicidal preparations and biopreparations to protect crops in agriculture.
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