Intron length polymorphism of tubulin genes as an effective tool for genetic plant differentiation
According to the scientific report at the meeting of the Presidium of the NAS of Ukraine, September 15, 2021
DOI:
https://doi.org/10.15407/visn2021.10.030Keywords:
molecular genetic markers, tubulin, TBP (tubulin base polymorphism), gene, intron length polymorphism, genetic differentiation, genotype, cultivar, population, speciesAbstract
A systematic assessment of the application possibility and effectiveness of one of the modern molecular marker systems based on the presence of an intron-specific DNA polymorphism of the tubulin family in plants (tubulin-based polymorphism, TBP) is performed. Because it is not yet widely used, this method requires verification of its effectiveness for genotyping plants at different taxonomic levels. The previous data are reviewed and new application examples of the TBP analysis in monocotyledonous and dicotyledonous plants assessment at the interspecies and intraspecies level are presented. The following plants are tested: wheat and barley cultivars, varieties and cultivars of camelina, cultivars of flax and Belarusian flax landraces, Antarctica island populations of hair grass, Crimean populations of aegilops, flax species, finger millet and Indian goosegrass, mistletoe subspecies. The high application efficiency of the β-tubulin gene intron length polymorphism assessment is shown for fingerprinting of various plant genotypes that can be used both for plant molecular genetics and for purposeful application in molecular selection, for example, during creating and testing of new varieties. The obtained results allow us to recommend this β-tubulin gene intron length evaluation method for the molecular genetic differentiation of monocotyledonous and dicotyledonous plant representatives because this method combines reliability and high output data obtaining speed with data analysis simplicity.
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