Development of Cultivation Technology for the Escherichia Coli Recombinant Strain Producing Arginine Deiminase of Mycoplasma Hominis

Authors

  • L.R. Fayura Institute for Cell Biology of NASU, Lviv
  • Yu.R. Boretsky Institute for Cell Biology of NASU, Lviv
  • Yu.V. Pynyaha Enzyme PJSC, Ladyzhyn
  • N.B. Martynyuk Enzyme PJSC, Ladyzhyn
  • V.V. Skorohod Enzyme PJSC, Ladyzhyn
  • A.A. Sybirny Institute for Cell Biology of NASU, Lviv

DOI:

https://doi.org/10.15407/scine10.04.029

Keywords:

arginine deiminase, bacteria Escherichia coli, Mycoplasma hominis, producer

Abstract

The Escherichia coli recombinant strain producing arginine deiminase of Mycoplasma hominis has been designed. Storage conditions that provide stabilization of most productive clones of the producer were found. Terms for cultivation of the arginine deiminase producer using bioreactors of different volume were optimized. Highly purified samples of arginine deiminase were obtained and their long-term storage conditions were selected.

References

Philip, R., Campbell, F., and Wheatley, D.N.: Arginine Deprivation, Growth Inhibition and Tumour Cell Death. Part 2. Enzymatic Degradation of Arginine in Normal and Malignant Cell Cultures. Br. J. Cancer, 88, 4, 213—283 (2003).

https://doi.org/10.1038/sj.bjc.6600681

Takaku, H., Matsumoto, M., Misawa, S., and Miyazaki, K.: Antitumor Activity of Arginine Deiminase from Mycoplasma Arginini and Its Growth – Inhibitory Mechanism. Jpn. J. Cancer Res., 86, 9, 840—846 (1995).

https://doi.org/10.1111/j.1349-7006.1995.tb03094.x

Sugimura, K., Ohno, T., Kusuyama, T., and Azuma, L.: High Sensitivity of Human Melanoma Cell Lines to the Growth Inhibitory Activity of Mycoplasmal Arginine Deiminase In Vitro. Melanoma Res., 2, 3, 191—196 (1992).

https://doi.org/10.1097/00008390-199209000-00007

Takaku, H., Takase, M., Abe, S. et al.: In Vivo Anti-Tumor Activity of Arginine Deiminase Purified from Mycoplasma Arginine. Int. J. Cancer, 51, 2, 244—249 (1992).

https://doi.org/10.1002/ijc.2910510213

Kuo, M.T., Savaraj, N., and Feun, L.G.: Targeted Cellular Metabolism for Cancer Chemotherapy with Recombinant Arginine-Degrading Enzymes. Oncotarget, 1, 4, 246—51 (2010).

Kubo, M., Nishitsuji, H., Kurihara, K. et al.: Suppression of Human Immunodeficiency Virus Type 1 Replication by Arginine Deiminase of Mycoplasma Arginine. J. Gen. Virol., 87, 6, 1589—1593 (2006).

https://doi.org/10.1099/vir.0.81549-0

Gong, H., Zolzer, F., von Recklinghhausen, G. et al.: Arginine Deiminase Inhibits Proliferation of Human Leukemia Cells More Potently than Asparaginase by Inducing Cell Cycle Arrest and Apoptosis. Leukemia, 14, 5, 826—829 (2000).

https://doi.org/10.1038/sj.leu.2401763

Ascierto, P.A., Scala, S., Castello, G. et al.: Pegylated Arginine Deiminase Treatment of Patients with Metastatic Melanoma: Results from Phase I and II Studies. J. Clin. Oncol., 23, 30, 7660—7668 (2005).

https://doi.org/10.1200/JCO.2005.02.0933

Ensor, C.M., Holtsberg, F.W., Bomalaski, J.S., and Clark, M.A.: Pegylated Arginine Deiminase (ADI-SS PEGv20,000 mw) Inhibits Human Melanomas and Hepatocellular Carcinomas In Vitro and In Vivo. Cancer Research, 62, 5443—5450 (2002).

Izzo, F., Marra, P., Beneduce, G. et al.: Pegylated Arginine Deiminase Treatment of Patients with Unresectable Hepatocellular Carcinoma: Results from Phase I/II Studies. J. Clin. Oncol., 22, 10, 1815—1822 (2004).

https://doi.org/10.1200/JCO.2004.11.120

Misawa, S., Aoshima, M., Takaku, H. et al.: High-Level Expression of Mycoplasma Arginine Deiminase in Escherichia coli and Its Efficient Renaturation as an Anti-Tumor Enzyme. Biotechnol., 36, 2, 145—155 (1994).

U.S. Patent, Pub. No.: US 2005/0129706 AI, MPC A61K 39/02, C12N 9/10. Modified Arginine Deiminase (2005).

Sambrook, J., Fritsh, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. New York: Cold Spring Harbor.

Sivashanmugam, A., Murray, V., Cui, C. et al.: Practical Protocols for Production of Very High Yields of Recombinant Proteins Using Escherichia coli. Protein Sci., 18, 5, 936—948 (2009).

https://doi.org/10.1002/pro.102

Studier, F.W.: Protein Production by Auto-Induction in High Density Shaking Cultures. Protein Expr. Purif., 41, 1, 207—234 (2005).

https://doi.org/10.1016/j.pep.2005.01.016

Fayura, L.R., Boretsky, Y.R., Pynyaha, Y.V. et al.: Improved Method for Expression and Isolation of the Mycoplasma Hominis Arginine Deiminase from the Recombinant Strain of Escherichia coli. J Biotechnol., 167(4), 420-6 (2013).

https://doi.org/10.1016/j.jbiotec.2013.06.025

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Published

2024-08-13

How to Cite

Fayura, L., Boretsky, Y., Pynyaha, Y., Martynyuk, N., Skorohod, V., & Sybirny, A. (2024). Development of Cultivation Technology for the Escherichia Coli Recombinant Strain Producing Arginine Deiminase of Mycoplasma Hominis. Science and Innovation, 10(4), 29–36. https://doi.org/10.15407/scine10.04.029

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Section

Research and Technical Innovative Projects of the National Academy of Sciences of Ukraine