Inhibition of multiple forms of carbonic anhydrases of spinach chloroplasts by Cu ions

Authors

  • A.V. Polishchuk M.G. Kholodhy Institute of Botany of the NAS of Ukraine, Kiev
  • A.V. Semenikhin Gogol Nizhyn State University, Ukraine
  • N.M. Topchyi M.G. Kholodhy Institute of Botany of the NAS of Ukraine, Kiev
  • E.K. Zolotareva M.G. Kholodhy Institute of Botany of the NAS of Ukraine, Kiev

DOI:

https://doi.org/10.15407/dopovidi2018.04.094

Keywords:

carbonic anhydrase, chloroplasts, copper ions, heavy metals, infrared gas analysis, Spinacia oleracea, thylakoid membranes

Abstract

The influence of Cu2+ on the carbonic anhydrase (CA) activity of various chloroplast fractions is studied. The stromal and thylakoid fractions are obtained after the osmotic destruction of intact chloroplasts washed out from cytosol components. Stromal CAs have been shown to be more sensitive to Cu2+ than the thylakoid CA. So, the halfmaximal inhibition (I50) of the CA activity of the stromal fraction is achieved at a concentration of 3 μM of Cu2+, whereas I50 at the inhibition of thylakoid CA was 10-20 μM Cu2+. In the presence of CuSO4, the distribution of the CA activity on the protein zones of the stromal and thylakoid fractions after the electrophoretic separation is changed: color intensity and the number of colored zones decrease depending on the Cu2+ concentration. The obtained data confirm the possibility of using CA of chloroplasts as a biomarker of the  environmental pollution.

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References

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Published

12.05.2024

How to Cite

Polishchuk, A., Semenikhin, A., Topchyi, N., & Zolotareva, E. (2024). Inhibition of multiple forms of carbonic anhydrases of spinach chloroplasts by Cu ions . Reports of the National Academy of Sciences of Ukraine, (4), 94–101. https://doi.org/10.15407/dopovidi2018.04.094

Issue

No. 4 (2018): Reports of the National Academy of Sciences of Ukraine

Section

Biochemistry

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