The gene expression pattern as a tool for assessment of components of microenvironment and response to anti-cancer therapy of prostate tumors
DOI:
https://doi.org/10.15407/dopovidi2019.04.086Keywords:
cancerassociated fibroblasts, pharmacological markers, prostate tumors, relative gene expression, tumor microenvironment, tumor-associated macrophagesAbstract
We have analyzed the putative value of the pattern of relative expression (RE) of several genes that might be involved in a response to anti-cancer therapy, namely AR, PTEN, COX2, FASN, HMGCR, LDLR, and CTLA4, in samples of prostate adenoma, adenocarcinoma, and the paired conventional normal tissues. We could propose three subtypes of adenocarcinomas that show the distinct pattern of expression of the above-mentioned genes, characteristics for (1) cancer-associated fibroblasts (CAFs), (2) tumor-associated macrophages (TAMs), and (3) markers of immune response. These groups correlate with the prostate cancer subtypes, that were determined earlier, based on the analysis of RE of the epithelial-to-mesenchymal cell transition (EMT) genes and prostate cancer-associated genes. Noteworthy, the highest correlation was found for genes characteristic of CAFs. This emphasizes the importance of the simultaneous analysis of genes, involved in various intercellular interactions between tumor cells and cells of tumor microenvironment, in prediction of efficacy of anti-cancer therapy. To confirm the presented data, the additional studies on a larger cohort of the prostate cancer patients are required.
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