Effects of temperature and Zn2+ ion concentration on the single-stranded polyC conformation in neutral solution
DOI:
https://doi.org/10.15407/dopovidi2014.12.158Keywords:
ion concentration, neutral solution, polyC, temperatureAbstract
The dependence of the polyC helicity degree on the temperature and the Zn2+ ion concentration in cacodylate buffer (pH 7, 0.015 M Na+) is studied by the methods of differential UV-spectroscopy and thermal denaturation. PolyC atoms coordinating Zn2+ ions were ascertained. Temperatures and zinc concentrations were established, at which the transition of polyC melted chains into an ordered structure being stabilized with intrachained connections N3−Zn2+−N3 was observed.
Downloads
References
Hartwig A. Pure Appl. Chem., 2000, 72, No 6: 1007– 1014. https://doi.org/10.1351/pac200072061007
Prasad A. S. Brit. Med. J., 2003, 326, No 7386: 409–10. https://doi.org/10.1136/bmj.326.7386.409
Sorokin V. A., Valeev V. A., Usenko E. L., Andrushchenko V. V. Int. J. Biol. Macromol., 2013, 61: 448–452. https://doi.org/10.1016/j.ijbiomac.2013.08.010
Galitskii V. A. Tsitologiia, 2009, 51: 388–397 (in Russian).
Vallee B. L., Gibson J. G. Blood., 1949, 4: 455–466.
Henkel R., Bittner J., Weber R. et al. Fertility and Sterility, 1999, 71, No 6: 1138–1143. https://doi.org/10.1016/S0015-0282(99)00141-7
Blagoi Yu. P., Galkin V. L., Gladchenko G. O. et al. Metal complexes of nucleic acids in solution, Kiev: Nauk. Dumka, 1991 (in Russian).
Borodavkin A. V., Budovskii E. I., Morozov Yu. V. et al. The electronic structure, UV absorption spectra and reactivity of nucleic acid components. In: The results of science and technology. Ser. Mol. biol., Moscow: VINITI, 1977, 14: 1–227 (in Russian).
Aoki K. Nucleosides, nucleotides and metal ions. In: Metalloproteins: Chemical properties and biological effects (Eds. S. Otsuka, T. Yamanaka), Amsterdam; Oxford; New York; Tokio: Elsevier, 1988: 457–490.
Sorokin V. A., Blagoi Yu. P., Valeev V. A., Antonova O. A. J. Inorg. Biochem., 1985, 24: 97–111. https://doi.org/10.1016/0162-0134(85)80002-7
Rifkind J. M., Shin Y. A., Heim J. M., Eichhorn G. L. Biopolymers, 1976, 15, No 9: 1879–1902. https://doi.org/10.1002/bip.1976.360151002
Kostiykov V. V. Stability of short hairpin structures of nucleic acids and their complexes with aromatic compounds (Unpublished dissertation). Sevastopol National Technical Univ., Sevastopol, 2007 (in Russian).
Vedenov A. A., Dykhne A. M., Phrank-Kamenetskiy M. D. Usp. phiz. nauk, 1971, 105, No 3: 479–519 (in Russian).
Landau L. D., Liphshits E. M. Statistical physics. Vol. 5, Moscow: Nauka, 1964 (in Russian).
Sorokin V. A., Valeev V. A., Usenko E. L. et al. Biophysics., 2007, 52, No 6: 545–557. https://doi.org/10.1134/S000635090706005X
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Reports of the National Academy of Sciences of Ukraine
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
