The cloning and peculiarities of the cellular localization of tobacco TPK potassium channels
DOI:
https://doi.org/10.15407/dopovidi2014.10.154Keywords:
cloning, potassium channels, tobacco, TPKAbstract
The characters of the subcellular localization of two TPK potassium channels from Tobacco were investigated. In order to create EYFP fusions, the cDNAs of NtTPK1a and NtTPK1b were cloned into specific vectors. The transient transformation of plant protoplast by created constructs revealed the differences in the subcellular localization for two TPK channels from tobacco. The localization of NtTPK1b in vesicular structures that are different from "typical" lytic vacuoles was detected.
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