The effect (phenomenon) of increasing the activity of monoclonal antibody I-1D to human D-dimer after heating
DOI:
https://doi.org/10.15407/dopovidi2015.09.091Keywords:
D-dimer, diagnostic test systems, fibrin, fibrinogen, monoclonal antibodies, thrombosisAbstract
The hybridoma which produces monoclonal antibody I-1D of the IgG1 class which specifically reacts only with human fibrin D-dimer without cross-reaction with fibrinogen and fibrin was obtained earlier by the authors. In this work, the significant increase of the binding affinity of this antibody to D-dimer after heating at 63 ºC during 10 min has been discovered. The other our D-dimerspecific monoclonal antibody III-3b doesn't change its properties after heating and was used as a control. Monoclonal antibody I-1D could be further used for the study of structural changes in a molecule of immunoglobulin.
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