Цитоскелет як внутрішньоклітинний регулятор і мішень для біологічно активних сполук
Стенограма доповіді на засіданні Президії НАН України 2 липня 2025 року
DOI:
https://doi.org/10.15407/visn2025.09.043Анотація
У доповіді наведено актуальні результати фундаментальних досліджень, проведених у ДУ «Інститут харчової біотехнології та геноміки НАН України» і спрямованих на розкриття ролі цитоскелета в забезпеченні структурної й сигнальної організації еукаріотичних клітин. Розглянуто можливості використання цитоскелета як мішені для пошуку природних і синтетичних біологічно активних речовин.
Як цитувати:
Ємець А.І. Цитоскелет як внутрішньоклітинний регулятор і мішень для біологічно активних сполук (за матеріалами доповіді на засіданні Президії НАН України 2 липня 2025 р.). Вісник НАН України. 2025. № 9. С. 43—49. https://doi.org/10.15407/visn2025.09.043
Посилання
Yemets A., Stelmakh O.A., Kundelchuk O.P., Blume Ya.B. Obtaining and analysis of isopropyl-N-phenyl carbamate resistant lines of Nicotiana species. Cell Biol. Int. 2003. 27(3): 307—310. https://doi.org/10.1016/S1065-6995(02)00348-7
Yemets A., Stelmakh O., Blume Ya. Effects of the herbicide isopropyl-N-phenyl carbamate on microtubules and MTOCs in lines of Nicotiana sylvestris resistant and sensitive to its action. Cell Biol. Int. 2008. 32(6): 623—629. https://doi.org/10.1016/j.cellbi.2008.01.012
Blume Ya., Yemets A., Sheremet Ya., Nyporko A., Sulimenko V., Sulimenko T., Draber P. Exposure of beta-tubulin regions defined by antibodies on Arabidopsis thaliana microtubule protofilament model and in the cells. BMC Plant Biol. 2010. 10: 29. https://doi.org/10.1186/1471-2229-10-29
Karpov P.A., Nadezhdina E.S., Yemets A.I., Matusov V.G., Nyporko A.Yu., Shashina N.Yu., Blume Ya.B. Bioinformatic search of plant microtubule- and cell cycle related serine-threonine protein kinases. BMC Genomics. 2010. 11(1): 14. https://doi.org/10.1186/1471-2164-11-S1-S14
Blume Ya., Yemets A., Sulimenko V., Sulimenko T., Chan J., Lloyd C., Dráber P. Tyrosine phosphorylation of plant tubulin. Planta. 2008. 229(1): 143—150. https://doi.org/10.1007/s00425-008-0816-z
Sheremet Ya.A., Yemets A.I., Vissenberg K., Verbelen J.-P., Blume Ya.B. Effects of inhibitors of serine/threonine protein kinases on Arabidopsis thaliana root morphology and microtubule organization in its cells. Cell Tiss. Biol. 2010. 4: 399—409. https://doi.org/10.1134/S1990519X10040139
Yemets A., Sheremet Ya., Vissenberg K., Van Orden J., Verbelen J.-P., Blume Ya.B. Effects of tyrosine kinase and phosphatase inhibitors on microtubules in Arabidopsis root cells. Cell Biol. Int. 2008. 32(6): 630—637. https://doi.org/10.1016/j.cellbi.2008.01.013
Yemets A.I., Krasylenko Yu.A., Lytvyn D.I., Sheremet Ya.A., Blume Ya.B. Nitric oxide signaling via cytoskeleton in plants. Plant Sci. 2011. 181(5): 545—554. https://doi.org/10.1016/j.plantsci.2011.04.017
Lytvyn D.I., Raynaud C., Yemets A.I., Bergounioux C., Blume Ya.B. Involvement of inositol biosynthesis and nitric oxide in the mediation of UV-B induced oxidative stress. Front. Plant Sci. 2016. 7: 430. https://doi.org/10.3389/fpls.2016.00430
Blume Ya.B., Krasylenko Yu.A., Demchuk O.M., Yemets A.I. Tubulin tyrosine nitration regulates microtubule organization in plant cells. Front. Plant Sci. 2013. 4: 530. https://doi.org/10.3389/fpls.2013.00530
Krasylenko Yu.A., Yemets A.I., Blume Ya.B. Nitric oxide synthase inhibitor L-NAME affects Arabidopsis root growth, morphology, and microtubule organization. Cell Biol. Int. 2019. 43(9): 1049—1055. https://doi.org/10.1002/cbin.10880
Olenieva V., Lytvyn D., Yemets A., Bergounioux C., Blume Y. Tubulin acetylation accompanies autophagy development induced by different abiotic stimuli in Arabidopsis thaliana. Cell Biol. Int. 2017. 43(9): 1056—1064. https://doi.org/10.1002/cbin.10843
Yemets A., Shadrina R., Blume R., Plokhovska S., Blume Ya. Autophagy formation, microtubule disorientation, and alteration of ATG8 and tubulin gene expression under simulated microgravity in Arabidopsis thaliana. npj Microgravity. 2024. 10(1): 31. https://doi.org/10.1038/s41526-024-00381-9
Blume Ya.B., Yemets A.I., Nyporko A.Yu., Baird W.V. Structural modeling of plant α-tubulin interaction with dinitroanilines and phosphoroamidates. Cell Biol. Int. 2003. 27(3): 171—174. https://doi.org/10.1016/S1065-6995(02)00298-6
Ozheredov S.P., Yemets A.I., Brytsun V.M., Ozheredova I.P., Lozynsky M.O., Blume Ya.B. Screening of new 2,4- and 2,6-dinitroaniline derivates for phytotoxicity and antimitotic activity. Cytol. Genet. 2009. 43(10): 297—304. https://doi.org/10.3103/S0095452709050016
Yemets A., Radchuk V., Bayer O., Bayer G., Pakhomov A., Baird V.W., Blume Ya.B. Development of transformation vectors based upon a modified plant α-tubulin gene as the selectable marker. Cell Biol. Int. 2008. 32(5): 566—570. https://doi.org/10.1016/j.cellbi.2007.11.012
Yemets A.I., Blume Ya.B., Kundelchuk O.P., Smertenko A.P., Solodushko V.A., Rudas V.A., Gleba Yu.Yu. Transfer of amiprophosmethyl resistance from Nicotiana plumbaginifolia mutant by somatic hybridisation. Theor. Appl. Genet. 2000. 100(6): 847—857. https://doi.org/10.1007/s001220051361
Yemets А.І., Baird W.V., Blume Ya.B. Modified tubulin genes as selectable markers for plant transformation. In: The Plant Cytoskeleton: Key Tool for Agro-Biotechnology. Springer, 2008. P. 435—454. https://doi.org/10.1007/978-1-4020-8843-8_21
Yemets А.І., Blume Ya.B. Antimitotic drugs for microprotoplast-mediated chromosome transfer in plant genomics, cell engineering and breeding. In: Blume Ya.B., Baird W.V., Yemets A.I., Breviario D. (eds) The Plant Cytoskeleton: Key Tool for Agro-Biotechnology. Springer, 2008. P. 419—434. https://doi.org/10.1007/978-1-4020-8843-8_20
Yemets A.I., Fedorchuk V.V., Blume Y.B. Enhancement of Agrobacterium-mediated transformation of plants using trifluoperazine and genistein—protein kinase inhibitors. Cytol. Genet. 2016. 50(2): 1—7. https://doi.org/10.3103/S0095452716010047
Mitrofanova I.V., Zilbervarg I.R., Yemets A.I., Mitrofanova O.V., Blume Ya.B. The effects of dinitroaniline and phosphoroamidate herbicides on polyploidization in vitro of Nepeta plants. Cell Biol. Int. 2003. 27(3): 229—231. https://doi.org/10.1016/s1065-6995(02)00317-7
Melnychuk O.V., Ozheredov S.P., Rakhmetov D.B., Yemets A.I., Blume Ya.B. Induction of polyploidy in giant Miscanthus (Miscanthus×giganteus Greef et Deu.). Proc. Latv. Acad. Sci. Sect. B: Nat. Exact. Appl. Sci. 2020. 74(3): 206—214. https://doi.org/10.2478/prolas-2020-0032
Melnychuk O.V., Ozheredov S.P., Rakhmetov D.B., Rakhmetova S.O., Yemets A.I., Blume Ya.B. The technology used for synthetic polyploid production of miscanthus as cellulosic biofuel feedstock. Open Agriculture Journal. 2020. 14: 164—173. https://doi.org/10.2174/1874331502014010164
Kustovskiy Ye., Karpov P., Blume Ya., Yemets A. Ivermectin affects Arabidopsis thaliana microtubules through predicted binding site of β-tubulin. Plant Physiol. Biochem. 2024. 206(1): 108296. https://doi.org/10.1016/j.plaphy.2023.108296
Kustovskiy Ye.O., Buziashvili A.Y., Ozheredov S.P., Blume Ya.B., Yemets A.I. β-Tubulin of Fusarium as a potential target for realization of antifungal activity of ivermectin. Cytol. Genet. 2024. 58: 1—10. https://doi.org/10.3103/S009545272401002X
Vus K., Tarabara U., Danylenko I., Pirko Ya., Krupodorova T., Yemets A., Blume Ya., Turchenko V., Klymchuk D., Smertenko P., Zhytniakivska O., Trusova V., Petrushenko S., Bogatyrenko S., Gorbenko G. Silver nanoparticles as inhibitors of insulin amyloid formation: A fluorescence study. J. Mol. Liq. 2021. 342: 117508. https://doi.org/10.1016/j.molliq.2021.117508
Garmanchuk L.V., Borovaya M.N., Nehelia A.O., Inomistova M., Khranovska N.M., Tolstanova G.M., Blume Ya.B., Yemets A.I. CdS quantum dots obtained by “green” synthesis: comparative analysis of toxicity and effects on the proliferative and adhesive activity of human cells. Cytol. Genet. 2019. 53(2): 132—142. https://doi.org/10.3103/S0095452719020026
Garmanchuk L., Borova M., Kapush O., Dzhagan V., Valakh M., Blume Ya., Yemets A. “Green” synthesis of CdTe quantum dots and their effect on human and animal cells. Cytol. Genet. 2023. 57(3): 229—238. https://doi.org/10.3103/S0095452723030040
Borovaya M., Naumenko A., Horiunova I., Plokhovska S., Blume Y., Yemets A. “Green” synthesis of Ag2S nanoparticles, study of their properties and bioimaging applications. Appl. Nanosci. 2020. 10(12): 4931—4940. https://doi.org/10.1007/s13204-020-01365-3
Borova M., Kapush O., Dzhagan V., Naumenko A., Blume Ya., Yemets A. Conjugates of biofabricated Ag2S quantum dots with monoclonal antibodies for microtubule visualization. Cytol. Genet. 2025. 59(4): 359—368. https://doi.org/10.3103/S0095452725040036
Yemets A., Plokhovska S., Pushkarova N., Blume Ya. Quantum dot-antibody conjugates for immunofluorescence studies of biomolecules and subcellular structures. J. Fluoresc. 2022. 32(5): 1713—1723. https://doi.org/10.1007/s10895-022-02968-5
