Development of Cultivation Technology for the Escherichia coli Recombinant Strain Producing Argininedeiminase of Mycoplasma hominis
DOI:
https://doi.org/10.15407/scin10.04.032Keywords:
arginine deiminase, producer, bacteria Escherichia coli, Mycoplasma hominis.Abstract
The recombinant Escherichia coli strain producing arginine deiminase of Mycoplasma hominis has been constructed. Storage conditions that provide stabilization of most productive clones of the producer were found. Terms for cultivation of the arginine deiminase producer using bioreactors of different volume were optimized. Highly purified samples of arginine deiminase were obtained and their longterm storage conditions were selected.
References
Philip, R., Campbell, F., and Wheatley, D.N.: Arginine Deprivation, Growth Inhibition and Tumour Cell Death. Part 2. Enzymatic Degradation of Arginine in Normal and Malignant Cell Cultures. Br. J. Cancer, 88, 4, 213-283 (2003).
https://doi.org/10.1038/sj.bjc.6600681
Takaku, H., Matsumoto, M., Misawa, S., and Miyazaki, K.: Antitumor Activity of Arginine Deiminase from Mycoplasma Arginini and Its Growth – Inhibitory Mechanism. Jpn. J. Cancer Res., 86, 9, 840-846 (1995).
https://doi.org/10.1111/j.1349-7006.1995.tb03094.x
Sugimura, K., Ohno, T., Kusuyama, T., and Azuma, L.: High Sensitivity of Human Melanoma Cell Lines to the Growth Inhibitory Activity of Mycoplasmal Arginine Deiminase In Vitro. Melanoma Res., 2, 3, 191-196 (1992).
https://doi.org/10.1097/00008390-199209000-00007
Takaku, H., Takase, M., Abe, S. et al.: In Vivo Anti-Tumor Activity of Arginine Deiminase Purified from Mycoplasma Arginine. Int. J. Cancer, 51, 2, 244-249 (1992).
https://doi.org/10.1002/ijc.2910510213
Kuo, M.T., Savaraj, N., and Feun, L.G.: Targeted Cellular Metabolism for Cancer Chemotherapy with Recombinant Arginine-Degrading Enzymes. Oncotarget, 1, 4, 246-51 (2010).
https://doi.org/10.18632/oncotarget.135
Kubo, M., Nishitsuji, H., Kurihara, K. et al.: Suppression of Human Immunodeficiency Virus Type 1 Replication by Arginine Deiminase of Mycoplasma Arginine. J. Gen. Virol., 87, 6, 1589-1593 (2006).
https://doi.org/10.1099/vir.0.81549-0
Gong, H., Zolzer, F., von Recklinghhausen, G. et al.: Arginine Deiminase Inhibits Proliferation of Human Leukemia Cells More Potently than Asparaginase by Inducing Cell Cycle Arrest and Apoptosis. Leukemia, 14, 5, 826-829 (2000).
https://doi.org/10.1038/sj.leu.2401763
Ascierto, P.A., Scala, S., Castello, G. et al.: Pegylated Arginine Deiminase Treatment of Patients with Metastatic Melanoma: Results from Phase I and II Studies. J. Clin. Oncol., 23, 30, 7660-7668 (2005).
https://doi.org/10.1200/JCO.2005.02.0933
Ensor, C.M., Holtsberg, F.W., Bomalaski, J.S., and Clark, M.A.: Pegylated Arginine Deiminase (ADI-SS PEGv20,000 mw) Inhibits Human Melanomas and Hepatocellular Carcinomas In Vitro and In Vivo. Cancer Research, 62, 5443-5450 (2002).
Izzo, F., Marra, P., Beneduce, G. et al.: Pegylated Arginine Deiminase Treatment of Patients with Unresectable Hepatocellular Carcinoma: Results from Phase I/II Studies. J. Clin. Oncol., 22, 10, 1815-1822 (2004).
https://doi.org/10.1200/JCO.2004.11.120
Misawa, S., Aoshima, M., Takaku, H. et al.: High-Level Expression of Mycoplasma Arginine Deiminase in Escherichia coli and Its Efficient Renaturation as an Anti-Tumor Enzyme. Biotechnol., 36, 2, 145-155 (1994).
https://doi.org/10.1016/0168-1656(94)90050-7
U.S. Patent, Pub. No.: US 2005/0129706 AI, MPC A61K 39/02, C12N 9/10. Modified Arginine Deiminase (2005).
Sambrook, J., Fritsh, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. New York: Cold Spring Harbor.
Sivashanmugam, A., Murray, V., Cui, C. et al.: Practical Protocols for Production of Very High Yields of Recombinant Proteins Using Escherichia coli. Protein Sci., 18, 5, 936-948 (2009).
https://doi.org/10.1002/pro.102
Studier, F.W.: Protein Production by Auto-Induction in High Density Shaking Cultures. Protein Expr. Purif., 41, 1, 207-234 (2005).
https://doi.org/10.1016/j.pep.2005.01.016
Fayura, L.R., Boretsky, Y.R., Pynyaha, Y.V. et al.: Improved Method for Expression and Isolation of the Mycoplasma Hominis Arginine Deiminase from the Recombinant Strain of Escherichia coli. J Biotechnol., 167(4), 420-6 (2013).
