Detection of Genes that Determine Maize Grain Quality Characteristics and Resistance to Stress Factors

Authors

  • O.V. Markovskyi Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Kyiv
  • M.O. Bannikova Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Kyiv
  • V.V. Borisova Institute of Steppe Zone Agriculture, NAAS of Ukraine. Dnipropetrovsk
  • T.V. Fedorenko Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Kyiv
  • B.V. Morgun Institute of Cell Biology and Genetic Engineering of NAS of Ukraine, Kyiv

DOI:

https://doi.org/10.15407/scin10.01.043

Keywords:

maize, transgenes, grain quality characteristics, primers, polymerase chain reaction (PCR).

Abstract

 200 experimental maize samples (Maize Company) were examined for the presence of genes that determine the quality characteristics of grain (wx and fl-2 genes), herbicide (bar (pat), epsps genes) and insect (cry-genes) resistance. The total DNA was extracted from maize living plant tissue. Primers to detect wx, fl-2, bar (pat), mepsps, CP4 epsps, cry1A(b), cry1F, cry1A.105, mcry3A, cry2Ab2, cry3Bb1, cry34Ab1, cry35Ab1 genes were designed and selected. Multiplex and Touchdown PCR were worked out. PCR amplification of certain sequences was carried out. No transgenes (bar (pat), mepsps, CP4 epsps, cry1A(b), cry1F, cry1A.105, mcry3A, cry2Ab2, cry3Bb1, cry34Ab1, cry35Ab1) were found among 200 analyzed experimental maize samples. At the same time, fl-2 gene was found in 41 samples, wx gene was found in 192 analyzed samples.

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Published

2024-06-12

Issue

Vol. 10 No. 1 (2014): Science and Innovation

Section

Research and Engineering Innovative Projects of the National Academy of Sciences of Ukraine