Development of Complex of Immunocytochemical and Molecular Genetic Technologies for Acute Leukemia Diagnostics and their Implementation into Clinical Practice
DOI:
https://doi.org/10.15407/scin9.01.044Keywords:
acute leukemias, cytomorphological diagnostics, immunophenotyping, reverse transcriptase polymerase chain reaction.Abstract
The state-of-the-art technologies of diagnosing acute leukemias (AL) based on the novel WHO classification have been developed. The major forms and cytological variants of hematoblastoses have been diagnosed precisely in 755 patients in Ukraine with one third of them suffering from AL. The primers for detecting the transcripts of chimerical genes bcr/abl (t(9;22)(q34;q11)); mll/af4 (t(4;11) (q21;q23)); mll/af9 (t(9;11)(p22;q23)) in reverse transcriptase polymerase chain reaction (RT-PCR) have been designed. RT-PCR has been used for differential diagnostics in AL patients.
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