Implementation of the Methods for Detection of Leaf Rust Resistance Genes in wheat Based on Carrying out Polymerase Chain Reaction

Authors

  • Ya.V. Pirko Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv
  • A.V. Karelov Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv, Institute of Plant Protection NAAS of Ukraine, Kyiv
  • N.A. Kozub Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv, Institute of Plant Protection NAAS of Ukraine, Kyiv
  • I.A. Sozinov Institute of Plant Protection NAAS of Ukraine, Kyiv
  • N.N. Pirko Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv
  • A.I. Yemets Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv
  • V.I. Korkhovy Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv
  • V.T. Koliuchiy V.M. Remeslo Myronivka Institute of Wheat, UAAS of Ukraine, Kiev region.
  • Ya.B. Blume Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv
  • A.A. Sozinov Institute of Cell Biology and Genetic Engineering NAS of Ukraine, Kyiv

DOI:

https://doi.org/10.15407/scin8.03.050

Keywords:

wheat, leaf rust (Puccinia triticina), leaf rust resistance, loci Lr34, polymerase chain reaction (PCR).

Abstract

The molecular genetic methods for identification of leaf rust (Puccinia triticina) resistance gene – Lr34, which is res ponsible for the generic non-specific resistance of wheat to the pathogen were developed. Polymerase chain reaction with codominant marker cssfr5 was carried out to determine the allelic status of the loci Lr34 in the 30 wheat varieties selected in Mironіvka Institute of Wheat of the National Academy of Agrarian Sciences of Ukraine and in the Institute of Plant Physiology and Genetics of National Academy of Sciences of Ukraine. The varieties containing allele Lr34(+), which confers resistance to leaf rust, represented 16,7 % from total number of all tested varieties. Obtained results were handed over to the Mironovka Institute of Wheat for use in breeding process.

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Published

2024-06-05

Issue

Vol. 8 No. 3 (2012): Science and Innovation

Section

Scientific Framework of Innovation Activities