Use of Endophytic Bacteria for Adaptation of in vitro grown Potato Plants to ex vitro Conditions and Protection of Planting Material from Phytopathogenes
DOI:
https://doi.org/10.15407/scin6.06.051Keywords:
endophytic bacteria, ex vitro adaptation, in vitro-grown potato, potato protectionAbstract
A scientific approval for the development of biotechnology for adaptation of potato plants in vitro and their natural immunnity priming by potato endophytic bacteria has been founded. Bacterization of healed potato by strains Methylobacterium radiotolerans IMBG290 and Pseudomonas putida IMBG294 at transferring to ex vitro conditions’ allowed to increase the plants engraftment and forming of valuable seedlings, and to avoid mandatory use of expensive pesticides. Minituber yield of seedlings obtained from inoculated cuttings exceeded control more than 50%. In addition, the number and weight of tubers increased. Priming potential of M. radiotolerans IMBG290 against potato pathogens with different strategies is a sort-specific. The positive effects of plant priming obtained after cuttings treatment during micropropagation preserved in the next generation.
References
Podolich O.V., Lytvynenko T.L., Voznjuk T.M. ta in. Vyjavlennja ugrupovan' endofitnyh bakterij v aseptychnyh roslynah kartopli pislja inokuljacii' Pseudomonas sp. IMBG 163. Nauk. visn. Uzhgorod. un-tu. 2006. No 18: 165−170 [in Ukrainian].
Frommel M.I., Novac J., Lazarovits G. Growth enhancement and developmental modifications in vitro grown potato (Solanum tuberosum spp. tuberosum) as affected by a nonfluorescent Pseudomonas sp. Plant Physiol. 1991. 96. P. 928-936.
https://doi.org/10.1104/pp.96.3.928
Petak A., Turjanica A., Kozyrovskaja N. Izuchenie vlijanija bakterij roda Klebsiella na regeneracionnye processy. Fiziologija i biohimija kul'turnyh rastenij. 1998. 28(4): 240-246 [in Russian].
Kozyrovska N., Petak A., Belavska N., et al. Inter relationships of Klebsiella with the plant: early events. Abstract Book 10th International Congress on Nitrogen Fixation (28 May - 3 June). S. Petersburg, Russia, 1995. P. 134.
Koval'chuk M.V., Rjazancev V.B., Kostjuk I.I., Kozyrovs'ka N.O. Prajmuvannja korysnymy bakterijamy v tehnologichnomu procesi klonal'nogo mikrorozmnozhennja kartopli. Visn. agrar. nauky. 2005. N 7. S. 43-45 [in Ukrainian].
Podolich O.V., Ardanov P.E., Voznyuk T.M., et al. Endophytic bacteria from potato in vitro activated by exogenic non-pathogenic bacteria. Biopolymers and Cell. 2007. 23, N 1. P. 21-28.
https://doi.org/10.7124/bc.000752
Podolich O., Laschevskyy V., Ovcharenko L., et al. Methylobacterium sp. resides in unculturable state in potato tissues in vitro and becomes culturable after induction by Pseudomonas fluorescens IMGB163. J. Appl. Microbiol. 2009. 116. P. 728-739.
https://doi.org/10.1111/j.1365-2672.2008.03951.x
Zaetz I. E., Kozyrovska N.O. Effect of a bacterial consortium on oxidative stress in soybean plants in cadmiumcontaminated soil. Biopolymers and Cell. 2008. 24, N 3. P. 246-253.
https://doi.org/10.7124/bc.0007A7
Koval'chuk M.V., Rjazancev V.B., Kostjuk I.I., Kozyrovs'ka N.O. Bioprajmuvannja u tehnologichnomu procesi klonal'nogo mikrorozmnozhennja kartopli (Solanum tuberosum L.). Visnyk agrarnoi' akademii' nauk. 2005. N 7. S. 43-45 [in Ukrainian].
Sziderics A.H., Rasche F., Trognitz F., et al. Bacterial endophytes contribute to abiotic stress ada ptation in pepper plants (Capsicum annuum L.). Can. J. Microbiol. 2007. 53. P. 1195-1202.
https://doi.org/10.1139/W07-082
Yang J., Kloepper J.W., Rye C.M. Rhizosphere bacteria help plants tolerate abiotic stress. Trends in Plant Science. 2009. 14. P. 1-4.
https://doi.org/10.1016/j.tplants.2008.10.004
Spoel S.H., Johnson J.S., Dong X. Regulation of tradeoffs between plant defenses against pathogens with different lifestyles. Proc Natl Acad Sci. USA. 2007. 104. P. 18842-18847.
https://doi.org/10.1073/pnas.0708139104
Trognitz F., Scherwinski K., Fekete A., et al. SchmittKopplin P., Trognitz B., Sessitsch A. Interaction between potato and the endophyte Burkholderia phytofirmans. Tagung der Vereinigung der Pfl anzenzüchter und Saatgutkaufleute Österreichs. 2008. P. 63-66.
Molinier J., Ries G., Zipfel C., Hohn B. Transgeneration memory of stress in plants. Nature. 2006. 442. P. 1046-1049.
https://doi.org/10.1038/nature05022
Boyko A., Kathiria K., Zemp F.J., et al. Transgenerational changes in the genome stability and methylation in pathogen-infected plants. Nucleic Acids Res. 2007. 35. P. 1714-1725.