Construction of the Recombinant Yeast Strain of Pichia stipitis with Improved Alcoholic Fermentation of Xylose
DOI:
https://doi.org/10.15407/scin6.06.045Keywords:
alcoholic fermentation, ethanol, Pichia stipitis, xylose, yeastAbstract
The modified form of the xylose reductase (XR) of Pichia stipitis with decreased affinity toward NADPН(H+) was created via site-specific mutagenesis. Recombinant strain of P. stipitis overexpressing the mutated XR characterized by 1,3-fold increase in productivity of xylose alcoholic fermentation was constructed. The rates of alcoholic fermentation of hydrolyzates of wheat bran and sawdust were also improved.
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