Construction of Riboflavin (Vitamin B2) Overproducers of the Yeast Candida Famata
DOI:
https://doi.org/10.15407/scin5.06.070Keywords:
Candida famata, overproducers, oversynthesis, riboflavin (vitamin B2), yeastAbstract
The stable riboflavin (RF) overproducers were isolated by means of amplification of the gene encoding positive regulator of RF synthesis into selected earlier C. famata flavinogenic strains. The medium components and cultivation conditions were optimized for maximal accumulation of product (RF).
References
Powers H.J. Riboflavin (vitamin B-2) and health. Amer. J. Clin. Nutr. 2003. Vol. 77. P. 1352-1360.
Vandamme E.J., Soetaert W. 2006. Personal care products via fermentation and biocatalysis processes. In: Biotechnology in Personal Care. Lad, R. (ed.). New York, USA: Taylor and Francis. P. 27-56.
Sanchez S., Demain A. Metabolic regulation and overproduction of primary metabolites. Microbial Biotechnology. 2008. Vol. 1, no 4. P. 283-319.
https://doi.org/10.1111/j.1751-7915.2007.00015.x
Stahmann K.P., Revuelta J.L., Seulberger H. Three biotechnical processes using Ashbya gossypii, Candida fama ta, or Bacillus subtilis compete with chemical riboflavin production. Microbiol. and Biotechnol. 2000. Vol. 53, no 5. P. 509-516.
https://doi.org/10.1007/s002530051649
Lim S.H., Choi J.S., Park E.Y. Microbial Production of Riboflavin Using Riboflavin Overproducers, Ashbya gossypii, Bacillus subtilis, and Candida famate: An Overview. Biotechnol. Bioprocess Eng. 2001. Vol. 6. P. 75-88.
https://doi.org/10.1007/BF02931951
Sybirnyj A.A., Fedorovych D.V., Borec'kyj Ju.R., Voronovs'kyj A.Ja. Mikrobnyj syntez flaviniv. Kyiv: Naukova dumka, 2006 [in Ukrainian].
Survase S.A., Bajaj I.B., Singhal R.S. Biotechnological Pro duction of Vitamins. Food Technol. Biotechnol. 2006. Vol. 44, no 3. P. 381-396.
Karos M., Vilarino C., Bollschweiler C., Revuelta J.L. A genomewide transcription analysis of a fungal riboflavin overproducer. J. Biotechnol. 2004. Vol. 113. P. 69-76.
https://doi.org/10.1016/j.jbiotec.2004.03.025
Dmytruk K.V., Voronovsky A.Y., Sibirny A.A. Insertion mutagenesis of the yeast Candida famata (Debaryomyces hansenii) by random integration of linear DNA fragments. Curr. Genet. 2006. Vol. 50, no 3. P. 183-191.
https://doi.org/10.1007/s00294-006-0083-0
Voronovsky A, Abbas C.A., Fayura L.R. et al. Development of a transformation system for the flavinogenic yeast. Candida famata. FEMS Yeast Res. 2002. Vol. 2, no 3. P. 381-388.
Sambrook J., Fritsh E.F., Maniatis T. Molecular Cloning: A La boratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York. 1989.
Patent Ukrai'ny na korysnu model' N 36164 vid 10.10.2008 [in Ukrainian].
Hyle J.W., Shaw R.J., Reines D. Functional distinctions bet ween IMP dehydrogenase genes in providing mycophenolate resistance and guanine prototrophy to yeast. J. Biol. Chem. 2003. Vol. 278, no 31. P. 28470-28478.
