Introduction of the Detection Methods of Genetic Modifications Content in Food, Feed and Cosmetic Products

Authors

  • Ya.B. Blume
  • M.O. Bannikova
  • P.A. Karpov
  • I.K. Komarnitsky
  • M.V. Kuchuk
  • B.V. Sorochinsky

Keywords:

detection, genetically modified plants, polymerase chain reaction

Abstract

Methods of genetically modified soybean, rape seeds, sugar beet and potato detection have been developed. To test above-mentioned cultivars and their products with the help of polymerase chain reaction the sets of oligonucleotide primers have been designed and synthesized. The manual to detect genetically modified components in plant raw materials, feed and food has been developed.

References

James C. Global status of commercialized biotech/GM crops. ISAAA Briefs. 2006. N 35 (International Service for the Acquisition of Agri-biotech Applications: Ithaca, New York).

Edwards K., Johnstone C., Thompson C. A simple and rapid method for the preparation of plant DNA for PCR analysis. Nucl. Acids Res. 1991, 19: 1349.

https://doi.org/10.1093/nar/19.6.1349

Cheung W.Y., Hubert N., Landry B.S. A simple and rapid DNA microextraction method for plant, animal and insect suitable for RAPD and other PCR analysis. PCR Meths. Applics. 1993, 3: 69—70.

Oard J.H., Dronovalli S. Rapid isolation of rice and maize DNA for analysis by random primer PCR. Plant Mol. Biol. Repts. 1992, 10: 236—241.

https://doi.org/10.1007/BF02668356

Huidet F. A powerful new technique to quickly prepare hundreds of plant extracts for PCR and RAPD analyses. Nucl. Acids Res. 1994, 22: 1771—1773.

Chunwongse J., Martin G.B., Tansklyi S.D. Pre-ermination genotypic screening using PCR amplification of half-seeds. Theor. Appl. Genet. 1993, vol.86: 694—698.

https://doi.org/10.1007/BF00222658

Wangn H., Qi M., Cutler J. A simple methods of preparing plant samples for PCR. Nucl. Acids Res. 1993, vol. 21: 4153—4154.

https://doi.org/10.1093/nar/21.17.4153

Naktinis V.I. Dva prostyh metoda vydelenija DNK iz razlichnyh istochnikov s primeneniem cetavlona. Biohimija. 1977, 42(10): 1783—1790 [in Russian].

Murray M.G., Thompson W.F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 1980, 8(19): 4321—4325.

https://doi.org/10.1093/nar/8.19.4321

Wurz A., Ruggeberg H., Brodmann P., Waiblinger H.U., Pietsch K. DNA-Extraktionsmethode fur den Nachweis gentechnisch veranderter Soja. Dtsch Lebensm Rundsch. 1998, vol. 94: 159—161.

Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory Press NY. 1989, N1, 2, 3. E. 3. 353 p.

Demeke T., Hunc P., Caswell K., Leung N., Chibbak R.N. Transgene inheritance and silencing in hexaploid spring wheat. Theor. Appl.Genet. 1999, vol. 99: 947—953.

https://doi.org/10.1007/s001220051401

Thompson C.J., Rao M.N., Tizard R., Crameri R., Davies J.E., Lauwereys M., Botterman J. Characterization of the herbicide_resistance gene bar from Streptomyces hydroscopicus. EMBO J. 1987, 6: 2519—2523.

Fischhoff D.A., Fuchs R.L., Lavrik P.B., McPherson S.A., Perlak F.J. Insect resistant tomato and potato plants. United States Patent 5495071. February 27, 1996.

Chen P.Y., Wang C.K., Soong S.C. and To K.Y. Complete sequence of the binary vector pBI121 and its application in cloning T_DNA insertion from transgenic plants. Mol. Breed. 2003, vol. 11: 287—293.

https://doi.org/10.1023/A:1023475710642

Published

2024-05-28

Issue

Section

Research and Engineering Innovative Projects of the National Academy of Sciences of Ukraine