Individual features of radiation-induced genomic instability in patients with glioblastoma

Authors

  • O.V. Zemskova Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine
  • O.Ya. Glavatsky Romodanov Neurosurgery Institute of the National Academy of Medical Sciences of Ukraine
  • D.A. Kurinnyi National Researh Center for Radiation Medicine of the NAMS of Ukraine, Kyiv
  • O.M. Demchenko National Researh Center for Radiation Medicine of the NAMS of Ukraine, Kyiv
  • S.R. Rushkovsky Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv

DOI:

https://doi.org/10.15407/dopovidi2020.04.091

Keywords:

apoptosis, Comet assay, glioblastoma, human peripheral blood lymphocytes culture, γ-irradiation

Abstract

Using the method of Comet assay under neutral conditions, the features of individual radiation-induced genome instability in patients with glioblastoma was studied. It was found that, in the culture of peripheral blood lymphocytes of two patients with pathomorphologically verified glioblastoma (patients No. 1 and No. 3), the frequency of comets with a high level of DNA damage significantly exceeded the values in the cultures of blood lymphocytes of the comparison group. After irradiation in a dose of 1.0 Gy, the frequency of cells with a high DNA damage rate was increased in lymphocyte cultures of patients No. 2 and No. 3 and decreased in patient No. 1. Frequency analysis of the distribution of individual “comets” depending on their levels of DNA damage revealed the presence of a significant pool of cells in the lymphocyte cultures of patients No. 2 and No. 3 which stopped di vision at the S stage of the cell cycle. After the irradiation, the frequency of such cells in patient No. 3 decreased significantly. It was noted that apoptotic activity in cultures of lymphocytes of neuro-oncological patients was significantly higher than in cultures of conditionally healthy volunteers.

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Published

28.03.2024

How to Cite

Zemskova, O. ., Glavatsky, O. ., Kurinnyi, D. ., Demchenko, O. ., & Rushkovsky, S. . (2024). Individual features of radiation-induced genomic instability in patients with glioblastoma . Reports of the National Academy of Sciences of Ukraine, (4), 91–98. https://doi.org/10.15407/dopovidi2020.04.091

Issue

No. 4 (2020): Reports of the National Academy of Sciences of Ukraine

Section

Biology

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